A number of surfactants, including representatives of the cationic, anionic, nonionic, and amphoteric groups, were studied for their effects on lactate dehydrogenase (LDH), protein content, and de novo synthesis, at concentrations ranging from 1 to 500 μg/ml medium, in a murine epidermal cell line (HEL/30). Protein synthesis, evaluated as [3H]leucine incorporation into cell proteins, was a more sensitive endpoint of toxicity than LDH leakage or protein content. Comparative surfactant toxicity followed the general order of cationic > anionic > nonionic > amphoteric. A good rank correlation was observed between their relative toxicity produced in vitro and the eye irritation produced in vivo in the Draize test. This sensitive and reproducible in vitro method may offer a means for screening potentially irritating water-soluble chemicals such as surfactants to quantify their toxicity.

Quantification of in vitro cytotoxicity of surfactants : Correlation with their eye irritation potential / M. Marinovich, E. Tragni, A. Corsini, C.L. Galli. - In: JOURNAL OF TOXICOLOGY-CUTANEOUS AND OCULAR TOXICOLOGY. - ISSN 0731-3829. - 9:3(1990), pp. 169-178.

Quantification of in vitro cytotoxicity of surfactants : Correlation with their eye irritation potential

M. Marinovich
Primo
;
E. Tragni
Secondo
;
A. Corsini
Penultimo
;
C.L. Galli
Ultimo
1990

Abstract

A number of surfactants, including representatives of the cationic, anionic, nonionic, and amphoteric groups, were studied for their effects on lactate dehydrogenase (LDH), protein content, and de novo synthesis, at concentrations ranging from 1 to 500 μg/ml medium, in a murine epidermal cell line (HEL/30). Protein synthesis, evaluated as [3H]leucine incorporation into cell proteins, was a more sensitive endpoint of toxicity than LDH leakage or protein content. Comparative surfactant toxicity followed the general order of cationic > anionic > nonionic > amphoteric. A good rank correlation was observed between their relative toxicity produced in vitro and the eye irritation produced in vivo in the Draize test. This sensitive and reproducible in vitro method may offer a means for screening potentially irritating water-soluble chemicals such as surfactants to quantify their toxicity.
Settore BIO/14 - Farmacologia
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/181084
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 20
  • ???jsp.display-item.citation.isi??? 26
social impact