Immunological and genomic analysis of the 'a' determinant was carried out in seven patients with concurrent HBsAg and anti-HBs, four of whom were immunized against hepatitis B virus at liver transplant, two with histologically characterized chronic hepatitis B virus infection, and one HBsAg healthy carrier. The immune reactivity of the HBsAg 'a' determinant was evaluated by binding to specific monoclonal antibodies, and the corresponding genomic sequence was studied by differential hybridization in microtiter plates and nucleotide sequence analysis. A double mutation generating an amino acid change (glycine to lysine) at residue 145, able to impair recognition by monoclonal antibodies, was observed in the post-transplant serum from one patient. No significant alteration of the 'a' determinant sequence or reactivity was detected in the other patients. Amino acid residue 145 appears therefore to be critical for the recognition by anti-HBs antibodies. A previously undescribed glycine to lysine substitution at this level interferes with the immune reactivity of the 'a' determinant.

Emergence of hepatitis B virus S gene mutant in a liver transplant recipient / E. CARIANI, A. RAVAGGI, E. TANZI, L. ROMANO', G. FIORDALISI, G. BELLATI, L. CACCAMO, D. GALMARINI, A. ALBERTINI, A.R. ZANETTI. - In: JOURNAL OF MEDICAL VIROLOGY. - ISSN 0146-6615. - 47:4(1995), pp. 410-415.

Emergence of hepatitis B virus S gene mutant in a liver transplant recipient

E. TANZI;L. ROMANO';A.R. ZANETTI
Ultimo
1995

Abstract

Immunological and genomic analysis of the 'a' determinant was carried out in seven patients with concurrent HBsAg and anti-HBs, four of whom were immunized against hepatitis B virus at liver transplant, two with histologically characterized chronic hepatitis B virus infection, and one HBsAg healthy carrier. The immune reactivity of the HBsAg 'a' determinant was evaluated by binding to specific monoclonal antibodies, and the corresponding genomic sequence was studied by differential hybridization in microtiter plates and nucleotide sequence analysis. A double mutation generating an amino acid change (glycine to lysine) at residue 145, able to impair recognition by monoclonal antibodies, was observed in the post-transplant serum from one patient. No significant alteration of the 'a' determinant sequence or reactivity was detected in the other patients. Amino acid residue 145 appears therefore to be critical for the recognition by anti-HBs antibodies. A previously undescribed glycine to lysine substitution at this level interferes with the immune reactivity of the 'a' determinant.
DNA enzyme immunoassay; Escape mutants; HBsAg 'a' determinant; Monoclonal antibodies; Polymerase chain reaction
Settore MED/42 - Igiene Generale e Applicata
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/181046
Citazioni
  • ???jsp.display-item.citation.pmc??? 4
  • Scopus 33
  • ???jsp.display-item.citation.isi??? ND
social impact