A simple and rapid RP-HPLC method for the direct determination of ceftazidime (a beta-lactam antibiotic) in agar (Mueller Hinton Agar-II) was developed. The method, characterised by good precision (C.V.less than or equal to 7.9%) and linearity in the 5-200 mu g/ml range (r(2) greater than or equal to 0.998), showed high recovery from agar (104+/-8%) and a sensitivity limit of 1.4 mu g/ml. The analytical procedure allowed the determination of the ''true'' antibiotic concentration in the agar matrix. In addition, the small volume sample may allow a precise evaluation of the antibiotic levels point by point in the agar plates necessary to study the kinetics of diffusion of ceftazidime.

Determination of ceftazidime concentration in Mueller Hinton agar by high-performance liquid chromatography / C. Arcelloni,M. Basile, R. Vaiani, P.A. Bonini, R. Paroni. - In: JOURNAL OF CHROMATOGRAPHY A. - ISSN 0021-9673. - 742:1-2(1996 Aug 23), pp. 121-126.

Determination of ceftazidime concentration in Mueller Hinton agar by high-performance liquid chromatography

R. Paroni
1996-08-23

Abstract

A simple and rapid RP-HPLC method for the direct determination of ceftazidime (a beta-lactam antibiotic) in agar (Mueller Hinton Agar-II) was developed. The method, characterised by good precision (C.V.less than or equal to 7.9%) and linearity in the 5-200 mu g/ml range (r(2) greater than or equal to 0.998), showed high recovery from agar (104+/-8%) and a sensitivity limit of 1.4 mu g/ml. The analytical procedure allowed the determination of the ''true'' antibiotic concentration in the agar matrix. In addition, the small volume sample may allow a precise evaluation of the antibiotic levels point by point in the agar plates necessary to study the kinetics of diffusion of ceftazidime.
Antibiotics; Ceftazidime; Muellar Hinton agar
Settore BIO/10 - Biochimica
Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica
JOURNAL OF CHROMATOGRAPHY A
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/181039
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