A new procedure for the assay of D-amino acid oxidase activity has been developed. alpha-Ketoisovaleric acid, derived from D-valine, was estimated by high-performance liquid chromatography after reaction with o-phenylenediamine to give the corresponding quinoxalinol derivative. alpha-Ketovaleric acid was used as an internal standard to ensure the reproducibility of the method. As an example of application, kidney cortex homogenates were analyzed for their D-amino acid oxidase activity. The advantages of the presented procedure for the determination of the enzymatic activity in biological samples compared with previously reported procedures are discussed.
HPLC determination of D-amino acid oxidase activity / P.A. Biondi, L. Guidotti, A. Negri, C.L. Secchi. - In: JOURNAL OF CHROMATOGRAPHY A. - ISSN 0021-9673. - 566:2(1991), pp. 377-382.
|Titolo:||HPLC determination of D-amino acid oxidase activity|
BIONDI, PIER ANTONIO (Primo)
NEGRI, ARMANDO (Penultimo)
SECCHI, CAMILLO LUIGI (Ultimo)
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
|Data di pubblicazione:||1991|
|Appare nelle tipologie:||01 - Articolo su periodico|