A new procedure for the assay of D-amino acid oxidase activity has been developed. alpha-Ketoisovaleric acid, derived from D-valine, was estimated by high-performance liquid chromatography after reaction with o-phenylenediamine to give the corresponding quinoxalinol derivative. alpha-Ketovaleric acid was used as an internal standard to ensure the reproducibility of the method. As an example of application, kidney cortex homogenates were analyzed for their D-amino acid oxidase activity. The advantages of the presented procedure for the determination of the enzymatic activity in biological samples compared with previously reported procedures are discussed.
HPLC determination of D-amino acid oxidase activity / P.A. Biondi, L. Guidotti, A. Negri, C.L. Secchi. - In: JOURNAL OF CHROMATOGRAPHY A. - ISSN 0021-9673. - 566:2(1991), pp. 377-382.
HPLC determination of D-amino acid oxidase activity
P.A. BiondiPrimo
;A. NegriPenultimo
;C.L. SecchiUltimo
1991
Abstract
A new procedure for the assay of D-amino acid oxidase activity has been developed. alpha-Ketoisovaleric acid, derived from D-valine, was estimated by high-performance liquid chromatography after reaction with o-phenylenediamine to give the corresponding quinoxalinol derivative. alpha-Ketovaleric acid was used as an internal standard to ensure the reproducibility of the method. As an example of application, kidney cortex homogenates were analyzed for their D-amino acid oxidase activity. The advantages of the presented procedure for the determination of the enzymatic activity in biological samples compared with previously reported procedures are discussed.Pubblicazioni consigliate
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