We investigated the presence of enzymes on the surface of Drosophila melanogaster spermatozoa that might bind to the carbohydrate residues of the egg shell. Spectrophotometric and fluorimetric studies were used on whole spermatozoa to assay galactosyltransferase and glycosidase activities. No galactosyltransferase is present on the sperm surface, whereas two glycosidases, b-N-acetylglucosaminidase (GlcNAc8ase) and a-mannosidase (Man8ase), have been evidenced. They have an optimal pH of 6–6.5 and 4, respectively. The same glycosidases were detected as soluble forms probably secreted by the seminal vesicle epithelium. We suggest that these enzymes might be involved in the recognition of a-mannose and b-N-acetylglucosamine residues present on the egg shell at the site of sperm entry.

Componenti della superficie di uovo e spermatozoo potenzialmente coinvolti nelle interazioni tra gameti in Drosophila melanogaster / M.E. Perotti, F. Cattaneo, M.E. Pasini, J.H.P. Hackstein. ((Intervento presentato al convegno Atti 57° Congresso Nazionale Unione Zoologica Italiana tenutosi a San Benedetto del Tronto nel 1996.

Componenti della superficie di uovo e spermatozoo potenzialmente coinvolti nelle interazioni tra gameti in Drosophila melanogaster

M.E. Pasini
Penultimo
;
1996

Abstract

We investigated the presence of enzymes on the surface of Drosophila melanogaster spermatozoa that might bind to the carbohydrate residues of the egg shell. Spectrophotometric and fluorimetric studies were used on whole spermatozoa to assay galactosyltransferase and glycosidase activities. No galactosyltransferase is present on the sperm surface, whereas two glycosidases, b-N-acetylglucosaminidase (GlcNAc8ase) and a-mannosidase (Man8ase), have been evidenced. They have an optimal pH of 6–6.5 and 4, respectively. The same glycosidases were detected as soluble forms probably secreted by the seminal vesicle epithelium. We suggest that these enzymes might be involved in the recognition of a-mannose and b-N-acetylglucosamine residues present on the egg shell at the site of sperm entry.
Settore BIO/06 - Anatomia Comparata e Citologia
Componenti della superficie di uovo e spermatozoo potenzialmente coinvolti nelle interazioni tra gameti in Drosophila melanogaster / M.E. Perotti, F. Cattaneo, M.E. Pasini, J.H.P. Hackstein. ((Intervento presentato al convegno Atti 57° Congresso Nazionale Unione Zoologica Italiana tenutosi a San Benedetto del Tronto nel 1996.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/180643
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