Rhodococcus sp. 1BN was isolated from a contaminated site and showed various biodegradative capabilities. Besides naphthalene, strain 1BN degraded medium- (C-6) and long-chain alkanes (C-16-C-28), benzene and toluene, alone or when the hydrocarbons were mixed in equal proportions. The nucleotide sequence of an alk polymerase chain reaction (PCR) fragment revealed a 59% nucleotide homology to the Pseudomonas oleovorans alkB gene. The nar fragments were highly homologous to genes coding for large and small subunits of cis-naphthalene 1,2-dioxygenase (narAa and narAb) and to cis-naphthalene dihydrodiol dehydrogenase (narB) from other rhodococci, The oxidation of indene to cis-(1S,2R)-1,2-dihydroxyindan by toluene-induced cells allows to hypothesize that strain 1BN also carries a toluene dioxygenase-like system.

Detection of genes for alkane and naphthalene catabolism in Rhodococcus sp. strain 1BN / V. Andreoni, S. Bernasconi, M. Colombo, J. van Beilen, L. Cavalca. - In: ENVIRONMENTAL MICROBIOLOGY. - ISSN 1462-2912. - 2:5(2000), pp. 572-577.

Detection of genes for alkane and naphthalene catabolism in Rhodococcus sp. strain 1BN

V. Andreoni;S. Bernasconi;M. Colombo;L. Cavalca
2000

Abstract

Rhodococcus sp. 1BN was isolated from a contaminated site and showed various biodegradative capabilities. Besides naphthalene, strain 1BN degraded medium- (C-6) and long-chain alkanes (C-16-C-28), benzene and toluene, alone or when the hydrocarbons were mixed in equal proportions. The nucleotide sequence of an alk polymerase chain reaction (PCR) fragment revealed a 59% nucleotide homology to the Pseudomonas oleovorans alkB gene. The nar fragments were highly homologous to genes coding for large and small subunits of cis-naphthalene 1,2-dioxygenase (narAa and narAb) and to cis-naphthalene dihydrodiol dehydrogenase (narB) from other rhodococci, The oxidation of indene to cis-(1S,2R)-1,2-dihydroxyindan by toluene-induced cells allows to hypothesize that strain 1BN also carries a toluene dioxygenase-like system.
dioxygenase; indene; biodegradation; bioconversion; genetics
Settore AGR/16 - Microbiologia Agraria
ENVIRONMENTAL MICROBIOLOGY
Article (author)
File in questo prodotto:
File Dimensione Formato  
1BN EM 2000.pdf

non disponibili

541 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/179520
Citazioni
  • ???jsp.display-item.citation.pmc??? 10
  • Scopus 51
  • ???jsp.display-item.citation.isi??? 49
social impact