A bimodular genetic fusion comprising a delivery module (scFv) and a capture module (SNAP) is proposed as a novel strategy for the site-specific covalent conjugation of targeting peptides to nanoparticles. An scFv mutant selective for HER2 tumor antigen is chosen as the targeting ligand. SNAP-scFv is immobilized on magnetofluorescent nanoparticles and its targeting efficiency against HER2-positive cells is assessed by flow cytometry and immunofluorescence. Copyright
Protein Oriented Ligation on Nanoparticles Exploiting O6-Alkylguanine-DNA Transferase (SNAP) Genetically Encoded Fusion / M. Colombo, S. Mazzucchelli, J.M. Montenegro, E. Galbiati, F.R.M. Corsi, W.J. Parak, D. Prosperi. - In: SMALL. - ISSN 1613-6810. - 8:10(2012), pp. 1492-1497.
Titolo: | Protein Oriented Ligation on Nanoparticles Exploiting O6-Alkylguanine-DNA Transferase (SNAP) Genetically Encoded Fusion | |
Autori: | COLOMBO, MIRIAM (Primo) MAZZUCCHELLI, SERENA (Secondo) | |
Parole Chiave: | bioconjugation; fusion proteins; magnetic nanoparticles; magnetofluorescence; tumor targeting | |
Settore Scientifico Disciplinare: | Settore MED/06 - Oncologia Medica Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica | |
Data di pubblicazione: | 2012 | |
Rivista: | ||
Tipologia: | Article (author) | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1002/smll.201102284 | |
Appare nelle tipologie: | 01 - Articolo su periodico |