Phytoplasmas are unculturable wall less bacteria involved in many plant diseases; in particular Flavescence dorée represents a severe threat to viticulture, because of its epidemic behavior in field. Here we present the application of the LDR technique, associated with hybridisation on universal arrays, as a quick and reliable tool for plant quarantine procedures and mandatory measures, in order to distinguish between different phytoplasmas' induced infections, compared to classical protocols in use, particularly PCR - RFLP assays.
Ligase detection reaction and universal arrays as a tool to detect grapevine infecting phytoplasmas / A. Frosini, P. Casati, P.A. Bianco, R. Bordoni, C. Consolandi, B. Castiglioni, A. Mezzeloni, E. Rizzi, C. Battaglia, G. Belli, L. Rossi Bernardi, G. De Bellis. - In: MINERVA BIOTECNOLOGICA. - ISSN 1120-4826. - 14:3-4(2002), pp. 265-267.
Ligase detection reaction and universal arrays as a tool to detect grapevine infecting phytoplasmas
P. Casati;P.A. Bianco;C. Consolandi;C. Battaglia;G. Belli;
2002
Abstract
Phytoplasmas are unculturable wall less bacteria involved in many plant diseases; in particular Flavescence dorée represents a severe threat to viticulture, because of its epidemic behavior in field. Here we present the application of the LDR technique, associated with hybridisation on universal arrays, as a quick and reliable tool for plant quarantine procedures and mandatory measures, in order to distinguish between different phytoplasmas' induced infections, compared to classical protocols in use, particularly PCR - RFLP assays.
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