Flavescence doree is a devastating disease of grapevine widespread in several countries in EU such as France, Italy and Spain. Genetic variability among 17 Italian and 3 French FD strains was investigated by RFLP analyses based on a fragment of the ribosomal protein operon and on the non-ribosomal DNA fragment FD9. RFLP analysis of the PCR amplified ribosomal protein fragment, coding for the 3' end of rpl22 and the entire rps3 genes, differentiated 4 rp-subgroups among the FD strains and 4 subgroups among the reference strains belonging to elm yellows group (16SrV). Sequencing and phylogenetic analysis of the same ribosomal protein DNA fragment validated the delineation of 4 distinct FD strain types derived by RFLP analyses. The results supported the differentiation based on analysis of the non-ribosomal DNA fragment FD9. The phylogenetic analysis further revealed relationships and a probable evolutionary trend among the FD strains and the other representatives of elm yellows group. All the FD strains together with the reference strains ALY, RuS and JWB formed a cluster very well distinct from the EY/ULW cluster. Moreover, ALY was shown to be more closely related to three FD strain types: the Lombardia/Piemonte, the French FD70, and the French FD88/ltalian FD-D strain clusters. (C) 2002 Elsevier Science Ltd. All rights reserved.
Genetic variability among flavescence dorée phytoplasmas from different origins in Italy and France / M. Martini, S. Botti, C. Marcone, C. Marzachi, P. Casati, P. Bianco, R. Benedetti, A. Bertaccini. - In: MOLECULAR AND CELLULAR PROBES. - ISSN 0890-8508. - 16:3(2002), pp. 197-208.
|Titolo:||Genetic variability among flavescence dorée phytoplasmas from different origins in Italy and France|
|Parole Chiave:||Flavescence dorée; Phylogeny; Phytoplasma; RFLP; Sequencing|
|Settore Scientifico Disciplinare:||Settore AGR/12 - Patologia Vegetale|
|Data di pubblicazione:||2002|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1006/mcpr.2002.0410|
|Appare nelle tipologie:||01 - Articolo su periodico|