TCR signal strength instructs αβ versus γδ lineage decision in immature T cells. Increased signal strength of γδTCR with respect to pre-TCR results in induction of the γδ differentiation program. Extracellular ATP evokes physiological responses through purinergic P2 receptors expressed in the plasma membrane of virtually all cell types. In peripheral T cells, ATP released upon TCR stimulation enhances MAPK activation through P2X receptors. We investigated whether extracellular ATP and P2X receptors signaling tuned TCR signaling at the αβ/γδ lineage bifurcation checkpoint. We show that P2X7 expression was selectively increased in immature γδ +CD25 + cells. These cells were much more competent to release ATP than pre-TCR-expressing cells following TCR stimulation and Ca 2+ influx. Genetic ablation as well as pharmacological antagonism of P2X7 resulted in impaired ERK phosphorylation, reduction of early growth response (Egr) transcripts induction, and diversion of γδTCR- expressing thymocytes toward the αβ lineage fate. The impairment of the ERK-Egr-inhibitor of differentiation 3 (Id3) signaling pathway in γδ cells from p2rx7 -/- mice resulted in increased representation of the Id3-independent NK1.1-expressing γδ T cell subset in the periphery. Our results indicate that ATP release and P2X7 signaling upon γδTCR expression in immature thymocytes constitutes an important costimulus in T cell lineage choice through the ERK-Egr-Id3 signaling pathway and contributes to shaping the peripheral γδ T cell compartment. Copyright
Purinergic P2X7 Receptor Drives T Cell Lineage Choice and Shapes Peripheral γδ Cells / M. Frascoli, J. Marcandalli, U. Schenk, F. Grassi. - In: JOURNAL OF IMMUNOLOGY. - ISSN 0022-1767. - 189:1(2012), pp. 174-180.
|Titolo:||Purinergic P2X7 Receptor Drives T Cell Lineage Choice and Shapes Peripheral γδ Cells|
GRASSI, FABIO MARIA (Ultimo)
|Settore Scientifico Disciplinare:||Settore BIO/13 - Biologia Applicata|
|Data di pubblicazione:||2012|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.4049/jimmunol.1101582|
|Appare nelle tipologie:||01 - Articolo su periodico|