Previous studies indicate that nuclear factor kappaB (NF-κB) transcription factor is deregulated and overexpressed in several human neoplasias. The aim of this study was to test the hypothesis that the NF-κB pathway may be involved in parathyroid tumorigenesis. For this purpose, we determined the level of NF-κB activity, evaluated as phosphorylation of the transcription subunit p65, its modulation by specific and non-specific agents and its impact on cyclin D1 expression. Phosphorylated p65 levels present in parathyroid neoplasias (n = 13) were significantly lower than those found in normal tissues (n = 3; mean optical density (OD) 0.19 ± 0.1 vs 0.4 ± 0.1, P = 0.007), but there was no significant difference between adenomas and secondary and multiple endocrine neoplasia type 1 (MEN1)-related hyperplasia. Conversely, MEN2A (Cys634Arg)-related parathyroid samples showed extremely high levels of phosphorylated p65 that exhibited a nuclear localization at immunohistochemistry (n = 3). Phosphorylated p65 levels negatively correlated with menin expression (r2 = 0.42, P = 0.05). Tumor necrosis factor-α (TNFα) caused a significant increase in phosphorylated p65 levels (183 ± 13.8% of basal) while calcium sensing receptor (CaR) agonists exerted a significant inhibition (19.2 ± 3.3% of basal). Although TNFα was poorly effective in increasing cyclin D1 expression, NF-κB blockade by the specific inhibitor BAY11-7082 reduced FCS-stimulated cyclin D1 by about 60%. Finally, the inhibitory effects of CaR and BAY11-7082 on cyclin D1 expression were not additive - by blocking NF-κB CaR activation did not induce a further reduction in cyclin D1 levels. In conclusion, the study demonstrated that in parathyroid tumors: (1) p65 phosphorylation was dramatically increased by RET constitutive activation and was negatively correlated with menin expression, (2) p65 phosphorylation was increased and reduced by TNFa and CaR agonists respectively, and (3) blockade of the NF-κB pathway caused a significant decrease in cyclin D1 expression.
Activity and function of the nuclear factor kappaB pathway in human parathyroid tumors / S. Corbetta, L. Vicentini, S. Ferrero, A. Lania, G. Mantovani, D. Cordella, P. Beck-Peccoz, A. Spada. - In: ENDOCRINE-RELATED CANCER. - ISSN 1351-0088. - 12:4(2005), pp. 929-937.
|Titolo:||Activity and function of the nuclear factor kappaB pathway in human parathyroid tumors|
CORBETTA, SABRINA LUIGIA (Primo)
VICENTINI, LUCIA (Secondo)
BECK PECCOZ, PAOLO LUIGI MARIA (Penultimo)
SPADA, ANNAMARIA (Ultimo)
|Settore Scientifico Disciplinare:||Settore MED/13 - Endocrinologia|
Settore MED/08 - Anatomia Patologica
Settore BIO/14 - Farmacologia
|Data di pubblicazione:||2005|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1677/erc.1.00970|
|Appare nelle tipologie:||01 - Articolo su periodico|