Zero (ZQ) and double (DQ) quantum 2D chemical shift selective and spin- echo 3D NMR imaging at microscopy resolution, has been applied to the morphological study of silkworm, Bombyx mori, during its metamorphosis. Attention has been focused on the evolution of the internal structure of the insect during its postembryonal life occurring through the larval, pupal and adult development. A major objective of this work was the characterization of the silk glands, responsible for synthesis and secretion of fibroin and sericin, through the changes of distribution and mobility of water, by imaging the water protons during postembryonal life stages. Moreover, alanine deriving from silk gland proteins was imaged during the last life stage of Bombyx mori.

Application of NMR microscopy to the morphological study of the silkworm, Bombyx mori, during its metamorphosis. / M. GUSSONI, F. GRECO, M. GUSSONI, R. CONSONNI, L. ZETTA. - In: MAGNETIC RESONANCE IMAGING. - ISSN 0730-725X. - 15(6):6(1997), pp. 693-700.

Application of NMR microscopy to the morphological study of the silkworm, Bombyx mori, during its metamorphosis.

M. GUSSONI
Primo
;
1997

Abstract

Zero (ZQ) and double (DQ) quantum 2D chemical shift selective and spin- echo 3D NMR imaging at microscopy resolution, has been applied to the morphological study of silkworm, Bombyx mori, during its metamorphosis. Attention has been focused on the evolution of the internal structure of the insect during its postembryonal life occurring through the larval, pupal and adult development. A major objective of this work was the characterization of the silk glands, responsible for synthesis and secretion of fibroin and sericin, through the changes of distribution and mobility of water, by imaging the water protons during postembryonal life stages. Moreover, alanine deriving from silk gland proteins was imaged during the last life stage of Bombyx mori.
Fibroin; Magnetic resonance imaging; Microscopy; Silkworm, Bombyx mori; ZQ and DQ NMRI
Settore BIO/13 - Biologia Applicata
Settore BIO/11 - Biologia Molecolare
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/176481
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