PDGF consists of two polypeptide chains, A and B, and all three possible dimers have been isolated from different sources. Human PDGF, essentially AB, porcine PDGF (BB) and recombinant PDGF-AA were tested on Swiss 3T3 fibroblasts for their ability to stimulate mitogenesis, phosphoinositide turnover and tyrosine phosphorylation of the PDGF receptor. When used in saturating amounts, the three isoforms were equally active in inducing mitogenesis. However, PDGF-AA was less active than AB and BB to induce the phosphorylation of the receptor and the turnover of phosphoinositides (30% and 50%, respectively). These findings suggest that, in Swiss 3T3 fibroblasts, PDGF receptors of the alpha-type are present in a slightly lower amount than beta-type. In addition, the two types of receptor appear to have similar physiological functions.
Effect of the different dimeric forms of the platelet-derived growth factor on cellular responses in mouse Swiss 3T3 fibroblasts / E. Sturani, R. Brambilla, L. Morello, M.G. Cattaneo, R. Zippel, L. Alberghina. - In: FEBS LETTERS. - ISSN 0014-5793. - 255:1(1989 Sep), pp. 191-195.
|Titolo:||Effect of the different dimeric forms of the platelet-derived growth factor on cellular responses in mouse Swiss 3T3 fibroblasts|
STURANI, EMMA PAOLA (Primo)
ZIPPEL, RENATA (Penultimo)
|Parole Chiave:||(Mouse fibroblast); Mitogenesis; Phosphoinositide; Platelet-derived growth factor isoform; Platelet-derived growth factor receptor; Tyrosine phosphorylation|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Data di pubblicazione:||set-1989|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/0014-5793(89)81089-0|
|Appare nelle tipologie:||01 - Articolo su periodico|