Amylin has been reported to have bone-conserving effects. In the present study we evaluated the possible activity of the peptide on human osteoblast-like (hOB) cells in primary culture. Amylin between 10(-9) and 10(-6) M, dose-dependently stimulated cell proliferation with a maximal effect (200%) at 10(-6) M. In addition, amylin increased osteocalcin production when hOB cells were exposed to 1,25(OH)2D3 (10(-8)M) but there was a nonsignificant upward trend on alkaline phosphatase activity. The present results suggest that amylin could be included among the group of peptides endowed with osteogenic activity.
|Titolo:||Effects of amylin on human osteoblast-like cells|
GUIDOBONO CAVALCHINI, FRANCESCA (Ultimo)
|Parole Chiave:||Alkaline phosphatase; Amylin; Osteoblast-like cells; Osteocalcin; Proliferation|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Data di pubblicazione:||1997|
|Digital Object Identifier (DOI):||10.1016/S0196-9781(97)00056-9|
|Appare nelle tipologie:||01 - Articolo su periodico|