The in vitro transformation, under anaerobic conditions, of 3- and 7-monosulfated and unsulfated bile acids, was studied in incubates of fecal flora from three healthy subjects. Chenodeoxycholic acid 7α-sulfate and ursodeoxycholic acid 7beta-sulfate were recovered unchanged, in all cultures, al the end of the incubation time. 3-Sulfated bile acids were metabolized in a different way by the three stool specimens. During the transformation of chenodeoxycholic acid 3-sulfate, desulfation, 7-dehydroxylation and 3-epimerization were observed. In contrast, 3-epimerization was not noticed when ursodeoxycholic acid 3-sulfate and lithocholic acid 3-sulfate were metabolized, the latter being principally transformed into ∆3-cholenic acid, probably by a bacterially mediated trans-elimination of sulfate group. The results obtained seem to prove that the presence of a SO3H group in 7-position usually hinders microbial transformations, which are not affected by a sulfate group in 3-position. Moreover, the 3-sulfated bile acids proved to be less sensitive to the microbial action than the corresponding un-sulfated acids, with exception of lithocholic 3-sulfate.
Transformation of sulfated bile acids by human intestinal microflora / N. Pacini, E. Albini, A. Ferrari, R. Zanchi, G. Marca, T. Bandiera. - In: ARZNEIMITTEL-FORSCHUNG. - ISSN 0004-4172. - 37:8(1987 Aug), pp. 983-987.
|Titolo:||Transformation of sulfated bile acids by human intestinal microflora|
|Parole Chiave:||Bile acids, sulphated ; Chenodeoxycholic acid 3- and 7-monosulfate ; Intestinal microflora, human Lithocholic acid 3-sulfate ; Sulfated bilee acids, in vitro transformation ; Urodeoxycholic acid 3- and 7-monosulfate|
|Settore Scientifico Disciplinare:||Settore AGR/16 - Microbiologia Agraria|
Settore MED/07 - Microbiologia e Microbiologia Clinica
|Data di pubblicazione:||ago-1987|
|Appare nelle tipologie:||01 - Articolo su periodico|