CONTEXT: Human oral mucosa is the combustion chamber of cigarette, but scanty evidence is available about the early smoke effects. OBJECTIVE: The present work aimed at evaluating from a morphological point of view whole smoke early effects on epithelial intercellular adhesion and keratinocyte terminal differentiation in a three-dimensional model of human oral mucosa. MATERIALS AND METHODS: Biopsies of keratinized oral mucosa of healthy nonsmoking women (n = 5) were collected. After culturing in a Transwell system, one fragment of each biopsy was exposed to the smoke of one single cigarette; the remnant represented the internal control. The distribution of epithelial differentiation markers (keratin-10, K10, and keratin-14, K14, for suprabasal and basal cells respectively), desmosomes (desmoglein-1, desmoglein-3), tight junctions (occludin), adherens junctions (E-cadherin, β-catenin), and apoptotic cells (p53, caspase 3) were evaluated by immunofluorescence. RESULTS: Quantitative analysis of K14 immunolabeling revealed an overexpression in the suprabasal layers as early as 3 h after smoke exposure, without impairment of the epithelial junctional apparatus and apoptosis induction. DISCUSSION AND CONCLUSION: These results suggested that the first significant response to cigarette smoke came from the basal and suprabasal layers of the human oral epithelium. The considered model maintained the three-dimensional arrangement of the human mucosa in the oral cavity and mimicked the inhalation/exhalation cycle during the exposure to cigarette smoke, offering a good possibility to extrapolate the reported observations to humans.

Acute effects of cigarette smoke on three-dimensional cultures of normal human oral mucosa / A. Gualerzi, M. Sciarabba, G. Tartaglia, C. Sforza, E. Donetti. - In: INHALATION TOXICOLOGY. - ISSN 0895-8378. - 24:6(2012), pp. 382-389. [10.3109/08958378.2012.679367]

Acute effects of cigarette smoke on three-dimensional cultures of normal human oral mucosa

A. Gualerzi
Primo
;
M. Sciarabba
Secondo
;
G. Tartaglia;C. Sforza
Penultimo
;
E. Donetti
Ultimo
2012

Abstract

CONTEXT: Human oral mucosa is the combustion chamber of cigarette, but scanty evidence is available about the early smoke effects. OBJECTIVE: The present work aimed at evaluating from a morphological point of view whole smoke early effects on epithelial intercellular adhesion and keratinocyte terminal differentiation in a three-dimensional model of human oral mucosa. MATERIALS AND METHODS: Biopsies of keratinized oral mucosa of healthy nonsmoking women (n = 5) were collected. After culturing in a Transwell system, one fragment of each biopsy was exposed to the smoke of one single cigarette; the remnant represented the internal control. The distribution of epithelial differentiation markers (keratin-10, K10, and keratin-14, K14, for suprabasal and basal cells respectively), desmosomes (desmoglein-1, desmoglein-3), tight junctions (occludin), adherens junctions (E-cadherin, β-catenin), and apoptotic cells (p53, caspase 3) were evaluated by immunofluorescence. RESULTS: Quantitative analysis of K14 immunolabeling revealed an overexpression in the suprabasal layers as early as 3 h after smoke exposure, without impairment of the epithelial junctional apparatus and apoptosis induction. DISCUSSION AND CONCLUSION: These results suggested that the first significant response to cigarette smoke came from the basal and suprabasal layers of the human oral epithelium. The considered model maintained the three-dimensional arrangement of the human mucosa in the oral cavity and mimicked the inhalation/exhalation cycle during the exposure to cigarette smoke, offering a good possibility to extrapolate the reported observations to humans.
Settore BIO/16 - Anatomia Umana
Settore BIO/17 - Istologia
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/174930
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