Bone-marrow samples were collected from 48 CAEV-seropositive, symptomless goats (30 kids, 18 adults). The samples were formalin-fixed and processed for histological examination. In addition, all samples were examined immunohistochemically with a monoclonal antibody (1A7) against the p27 capsid protein of maedi-visna virus, an antibody which cross-reacts with the Ca-p27 of CAEV. Samples from 16 goats (10/30 kids, 6/18 adults) showed positive immunolabelling of bone-marrow stromal cells (fibrocytes, endothelial cells and adipocytes) and of scattered macrophages, whereas haematopoietic cells were negative. The detection of viral Ca-p27 protein in bone-marrow fibrocytes was consistent with previous in-vitro studies which indicated that such cells are semi-permissive for CAEV infection. It is speculated that bone-marrow stromal cells represent a viral reservoir in symptomless animals

Immunohistochemical detection of p27-CAEV capside protein in bone marrow cells of sieropositive goats / P. Grossi, C. Giudice, I. Bertoletti, G. Cioccarelli, E. Brocchi, G. Cammarata, D. Gelmetti. - In: JOURNAL OF COMPARATIVE PATHOLOGY. - ISSN 0021-9975. - 133:2-3(2005), pp. 197-200. [10.1016/j.jcpa.2005.01.009]

Immunohistochemical detection of p27-CAEV capside protein in bone marrow cells of sieropositive goats

C. Giudice
Secondo
;
2005

Abstract

Bone-marrow samples were collected from 48 CAEV-seropositive, symptomless goats (30 kids, 18 adults). The samples were formalin-fixed and processed for histological examination. In addition, all samples were examined immunohistochemically with a monoclonal antibody (1A7) against the p27 capsid protein of maedi-visna virus, an antibody which cross-reacts with the Ca-p27 of CAEV. Samples from 16 goats (10/30 kids, 6/18 adults) showed positive immunolabelling of bone-marrow stromal cells (fibrocytes, endothelial cells and adipocytes) and of scattered macrophages, whereas haematopoietic cells were negative. The detection of viral Ca-p27 protein in bone-marrow fibrocytes was consistent with previous in-vitro studies which indicated that such cells are semi-permissive for CAEV infection. It is speculated that bone-marrow stromal cells represent a viral reservoir in symptomless animals
Bone-marrow stromal cells; Caprine arthritis-encephalitis; Goat; Lentivirus; Retrovirus; Viral infection
Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/17454
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