Background and aims. We generated a computerized morphometric model to evaluate and quantify the morphological features in large regenerative nodules (LRN), high-grade dysplastic nodules (HGDN) and hepatocellular carcinoma (HCC). Methods. Sixteen LRN, 10 HGDN and 16 HCC in HCV-cirrhotic livers, were stained with H&E, smooth muscle actin, CD34, CD31 and reticulin to evaluate volume and surface fractions. Results. On H&E stains, the features most discriminatory between LRN, HGDN and HCC were volume fraction and number of hepatocytes nuclei in unit volume, and hepatocyte nuclear/cytoplasmic ratio. On immunohistochemistry, volume fractions of capillarised sinusoids, capillary units and isolated arteries were significantly different among all groups and highest in HCC; surface fraction of reticulin was markedly decreased in HCC. Conclusions. Our morphometric model is an objective method of quantification of the morphological changes of the nodular lesions and it could be applied in studies involving histological evaluation of the spectrum of nodular lesions arising in the cirrhotic liver.

Morphometric analysis of hepatocellular nodular lesions in HCV cirrhosis / M. Vertemati, C. Moscheni, D. Petrella, L. Lamperti, M. Cossa, M. Gambacorta, M. Goffredi, L. Vizzotto. - In: PATHOLOGY RESEARCH AND PRACTICE. - ISSN 0344-0338. - 208:4(2012 Apr), pp. 240-244.

Morphometric analysis of hepatocellular nodular lesions in HCV cirrhosis

M. Vertemati
;
C. Moscheni
Secondo
;
L. Lamperti;M. Cossa;M. Goffredi
Penultimo
;
L. Vizzotto
Ultimo
2012-04

Abstract

Background and aims. We generated a computerized morphometric model to evaluate and quantify the morphological features in large regenerative nodules (LRN), high-grade dysplastic nodules (HGDN) and hepatocellular carcinoma (HCC). Methods. Sixteen LRN, 10 HGDN and 16 HCC in HCV-cirrhotic livers, were stained with H&E, smooth muscle actin, CD34, CD31 and reticulin to evaluate volume and surface fractions. Results. On H&E stains, the features most discriminatory between LRN, HGDN and HCC were volume fraction and number of hepatocytes nuclei in unit volume, and hepatocyte nuclear/cytoplasmic ratio. On immunohistochemistry, volume fractions of capillarised sinusoids, capillary units and isolated arteries were significantly different among all groups and highest in HCC; surface fraction of reticulin was markedly decreased in HCC. Conclusions. Our morphometric model is an objective method of quantification of the morphological changes of the nodular lesions and it could be applied in studies involving histological evaluation of the spectrum of nodular lesions arising in the cirrhotic liver.
Morphometry; Hepatic nodules; Cirrhosis; Immunohistochemistry
Settore BIO/16 - Anatomia Umana
Settore BIO/17 - Istologia
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/173837
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