The leitmotiv of mitochondrial genome (mtDNA) evolution in ascidians is the hypervariability of many genomic features (such as gene order, nucleotide substitution rate and tRNA gene content) even at short phylogenetic distances. As consequence, the ascidian mtDNA has been proved to unambiguously discriminate between two cryptic species of Ciona intestinalis. Here, we describe the mtDNA of a model species, the colonial ascidian Botryllus schlosseri. Two specimens, one from California and one from Italy, have been sequenced. We found that the mtDNA of B. schlosseri has a novel gene order, completely different from other ascidians, and encodes for peculiar tRNAlike structures. Surprisingly, the sequence divergence between the two specimens is up to one order of magnitude higher (depending on the functional mt region) than the one measured in other ascidian intra-species comparisons. However, this value is lower than the one measured within genera, and between the two cryptic C. intestinalis species. Thus, based on mtDNA, the Californian and Italian B. schlosseri specimens appear to belong to a single species that is characterized by a remarkably high nucleotide substitution rate.

Mitogenomics reveals a remarkably high intra-species substitution rate in the ascidian Botryllus schlosseri / F. Griggio, A. Voskoboynik, F. Iannelli, D. Pushkarev, C. Gissi. ((Intervento presentato al 6. convegno International Tunicate Meeting tenutosi a Montreal nel 2011.

Mitogenomics reveals a remarkably high intra-species substitution rate in the ascidian Botryllus schlosseri

F. Griggio
Primo
;
F. Iannelli;C. Gissi
Ultimo
2011

Abstract

The leitmotiv of mitochondrial genome (mtDNA) evolution in ascidians is the hypervariability of many genomic features (such as gene order, nucleotide substitution rate and tRNA gene content) even at short phylogenetic distances. As consequence, the ascidian mtDNA has been proved to unambiguously discriminate between two cryptic species of Ciona intestinalis. Here, we describe the mtDNA of a model species, the colonial ascidian Botryllus schlosseri. Two specimens, one from California and one from Italy, have been sequenced. We found that the mtDNA of B. schlosseri has a novel gene order, completely different from other ascidians, and encodes for peculiar tRNAlike structures. Surprisingly, the sequence divergence between the two specimens is up to one order of magnitude higher (depending on the functional mt region) than the one measured in other ascidian intra-species comparisons. However, this value is lower than the one measured within genera, and between the two cryptic C. intestinalis species. Thus, based on mtDNA, the Californian and Italian B. schlosseri specimens appear to belong to a single species that is characterized by a remarkably high nucleotide substitution rate.
English
3-lug-2011
Settore BIO/11 - Biologia Molecolare
Presentazione
Intervento richiesto
Sì, ma tipo non specificato
International Tunicate Meeting
Montreal
2011
6
Convegno internazionale
F. Griggio, A. Voskoboynik, F. Iannelli, D. Pushkarev, C. Gissi
Mitogenomics reveals a remarkably high intra-species substitution rate in the ascidian Botryllus schlosseri / F. Griggio, A. Voskoboynik, F. Iannelli, D. Pushkarev, C. Gissi. ((Intervento presentato al 6. convegno International Tunicate Meeting tenutosi a Montreal nel 2011.
Prodotti della ricerca::14 - Intervento a convegno non pubblicato
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/173753
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