Formation of multinucleated giant cells (MGCs) by macrophage fusion is a typical cytopathic effect of lentiviral replication in caprine monocytes and MGC formation from cultured caprine peripheral blood mononuclear cells (PBMCs) has been considered to be diagnostic for small ruminant lentivirus (SRLV) infection. In this study, formation of MGCs was observed after 7–14 days when PBMCs were cultured from healthy goats free from SRLV infection. These MGCs expressed tartrate-resistant acid phosphatase, calcitonin receptor, integrin aVb3, cathepsin K and matrix metalloproteinase 9 and were able to resorb bone in vitro in the absence of RANKL and macrophage colony stimulating factor, consistent with an osteoclast phenotype.
Multinucleated giant cells with an osteoclast phenotype derived from caprine peripheral blood mononuclear cells / G. Pisoni, P. D’Amelio, F. Sassi, G. Manarolla, L. Scaccabarozzi, C. Locatelli, N. Mazzocchi, M. Baruscotti, D. DiFrancesco, P. Moroni. - In: THE VETERINARY JOURNAL. - ISSN 1090-0233. - 189:3(2011 Sep), pp. 361-363.
|Titolo:||Multinucleated giant cells with an osteoclast phenotype derived from caprine peripheral blood mononuclear cells|
|Parole Chiave:||Caprine ; multinucleated giant cells ; peripheral blood mononuclear cells ; osteoclast|
|Settore Scientifico Disciplinare:||Settore VET/05 - Malattie Infettive degli Animali Domestici|
Settore BIO/09 - Fisiologia
|Data di pubblicazione:||set-2011|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.tvjl.2010.07.016|
|Appare nelle tipologie:||01 - Articolo su periodico|