Blood typing is an essential tool in clinical feline practice to prevent serious transfusion and neonatal isoerythrolysis reactions. An AB blood group system has been recognized in cats. The purpose of this study was to evaluate the performance of a currently available column gel agglutination technique for feline blood group typing. Each sample was blood typed in parallel using both a commercial column gel agglutination technique for feline blood group typing (ID Gel-Test Micro Typing System, Diamed) and an agglutination assay card method (RapidVet-H feline, Agrolabo). If there were discrepancies between the results obtained from the two methods, blood type was confirmed with a back-typing hemoagglutination test. The gel agglutination technique was easy to perform, reproducible, and provided semiquantitative, clear and stable results. We obtained concordant results with the card method and the column gel agglutination technique for feline blood group typing with the exception of type AB cats. In type AB cats, the gel agglutination technique was more sensitive than the agglutination on card method that tended to mis-type AB cats as B.

Assessment of a gel column technique for feline blood typing / D. Proverbio, E. Spada, L. Baggiani, R. Perego. - In: VETERINARY RESEARCH COMMUNICATIONS. - ISSN 0165-7380. - 33:Suppl. 1(2009 Sep), pp. 201-203. ((Intervento presentato al 62. convegno Convegno nazionale SISVet tenutosi a San Benedetto del Tronto nel 2008.

Assessment of a gel column technique for feline blood typing

D. Proverbio;E. Spada;L. Baggiani;R. Perego
2009-09

Abstract

Blood typing is an essential tool in clinical feline practice to prevent serious transfusion and neonatal isoerythrolysis reactions. An AB blood group system has been recognized in cats. The purpose of this study was to evaluate the performance of a currently available column gel agglutination technique for feline blood group typing. Each sample was blood typed in parallel using both a commercial column gel agglutination technique for feline blood group typing (ID Gel-Test Micro Typing System, Diamed) and an agglutination assay card method (RapidVet-H feline, Agrolabo). If there were discrepancies between the results obtained from the two methods, blood type was confirmed with a back-typing hemoagglutination test. The gel agglutination technique was easy to perform, reproducible, and provided semiquantitative, clear and stable results. We obtained concordant results with the card method and the column gel agglutination technique for feline blood group typing with the exception of type AB cats. In type AB cats, the gel agglutination technique was more sensitive than the agglutination on card method that tended to mis-type AB cats as B.
Agglutination ; blood groups ; cat ; card ; column gel
Settore VET/08 - Clinica Medica Veterinaria
VETERINARY RESEARCH COMMUNICATIONS
Società italiana dele scienze veterinarie
SISVet
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/171661
Citazioni
  • ???jsp.display-item.citation.pmc??? 2
  • Scopus 12
  • ???jsp.display-item.citation.isi??? 12
social impact