Objective: We aimed to characterize the fibroblast phenotype of patients by analyzing gene and protein expression of cleft lip and/or cleft palate fibroblasts in relation to collagen turnover and extracellular matrix remodeling. Patients: Human palatal fibroblasts were obtained from three healthy subjects without cleft lip and/or cleft palate and from three subjects with nonsyndromic cleft lip and/or cleft palate. Collagen turnover-related gene and protein expression were analyzed by real-time polymerase chain reaction, Western and dot blots, and sodium dodecyl sulfate zymography. Results: Cleft lip and/or cleft palate fibroblasts, compared with controls, displayed a down-regulation of collagens type I and III messenger RNA (p < .0001 and p < .001, respectively) but an opposite tendency to increase protein levels. Cleft lip and/or cleft palate cells had higher lysyl hydroxylase-2b messenger RNA levels expressed in relation to collagen type I messenger RNA, down-regulated matrix metalloproteinase-1, tissue inhibitor of matrix metalloproteinase-1, and Secreted Protein Acidic and Rich in Cysteine messenger RNA (p < .0001 and p < .01, respectively). Pro-matrix metalloproteinase-1 tended to decrease, and pro-matrix metalloproteinase-2 and -9 were down-regulated (p < .01, p < .05, respectively), as was Secreted Protein Acidic and Rich in Cysteine protein expression (p < .05). Conclusions: Our results suggest that the cleft lip and/or cleft palate fibroblast phenotype is characterized by a tendency toward interstitial collagen deposition due to posttranslational modifications, such as decreased collagen degradation by matrix metalloproteinases and increased collagen cross-links. These findings may contribute to the knowledge of the cleft lip and/or cleft palate fibroblast phenotype and may be useful to the surgeon when considering the potential wound contraction and subsequent undesired scarring in cleft lip and/or cleft palate ocurring after the surgical closure of a cleft palate.
New insights in collagen turnover in orofacial cleft patients / N.Gagliano, F. Carinci, C. Moscheni, C. Torri, F. Pezzetti, L. Scapoli, M. Martinelli, M. Gioia, G. Stabellini. - In: CLEFT PALATE-CRANIOFACIAL JOURNAL. - ISSN 1055-6656. - 47:4(2010 Jul), pp. 393-399.
|Titolo:||New insights in collagen turnover in orofacial cleft patients|
GAGLIANO, NICOLETTA (Corresponding)
|Parole Chiave:||collagen turnover; lysyl hydroxylase; matrix metalloproteinases; orofacial cleft; SPARC|
|Settore Scientifico Disciplinare:||Settore BIO/16 - Anatomia Umana|
Settore BIO/17 - Istologia
|Data di pubblicazione:||lug-2010|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1597/07-196.1|
|Appare nelle tipologie:||01 - Articolo su periodico|