Flowering of Arabidopsis is induced by long summer days (LDs). The transcriptional regulator CONSTANS (CO) promotes flowering, and its transcription is increased under LDs. We systematically misexpressed transcription factors in companion cells and identified several DOF proteins that delay flowering by repressing CO transcription. Combining mutations in four of these, including CYCLING DOF FACTOR 2 (CDF2), caused photoperiod-insensitive early flowering by increasing CO mRNA levels. CO transcription is promoted to differing extents by GIGANTEA (GI) and the F-box protein FKF1. We show that GI stabilizes FKF1, thereby reducing CDF2 abundance and allowing transcription of CO. Despite the crucial function of GI in wild-type plants, introducing mutations in the four DOF-encoding genes into gi mutants restored the diurnal rhythm and light inducibility of CO. Thus, antagonism between GI and DOF transcription factors contributes to photoperiodic flowering by modulating an underlying diurnal rhythm in CO transcript levels.
Arabidopsis DOF transcription factors act redundantly to reduce CONSTANS expression and are essential for a photoperiodic flowering response / F. Fornara, K. Panigrahi, L. Gissot, N. Sauerbrunn, M. Rühl, G. Coupland. - In: DEVELOPMENTAL CELL. - ISSN 1534-5807. - 17:1(2009 Jul 21), pp. 75-86.
|Titolo:||Arabidopsis DOF transcription factors act redundantly to reduce CONSTANS expression and are essential for a photoperiodic flowering response|
FORNARA, FABIO (Primo)
|Parole Chiave:||Flowers ; Arabidopsis Proteins ; Plants, Genetically Modified ; Photoperiod; DNA-Binding Proteins ; RNA, Messenger ; Circadian Rhythm ; Transcription Factors ; Repressor Proteins ; Arabidopsis ; Gene Expression Regulation, Plant ; Mutation ; Signal Transduction|
|Settore Scientifico Disciplinare:||Settore BIO/01 - Botanica Generale|
Settore BIO/11 - Biologia Molecolare
|Data di pubblicazione:||21-lug-2009|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.devcel.2009.06.015|
|Appare nelle tipologie:||01 - Articolo su periodico|
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