The new tumor-specific antigens Bcr/Abl-OOF, identified in Philadelphia chromosome (Ph)-positive leukemia cells, are derived from an alternative splicing event involving BCR exons 1, 13, or 14 and ABL exons 4 and 5. The COOH-terminus of these transcription products contain an amino acid portion derived from an out-of-frame (OOF) reading of the ABL gene; these variants are expressed in Ph-positive chronic myelogenous leukemia (CML) and acute lymphocytic leukemia patients. Previously, we confirmed the presence of out-of-frame peptide-specific T cells in the peripheral blood of CML patients with the ability to lyse primary autologous CML cells. We also demonstrated that the out-of-frame Abl portion was immunogenic in HLA-A2.1 transgenic mice. Here we describe the production and characterization of monoclonal antibody 1D8G8, a new tool for localization and functional studies of the tumor antigen Bcr/Abl-OOF. This antibody recognizes the out-of-frame protein portion of the native full-length Bcr/Abl-OOF protein expressed in cells transiently transfected, as demonstrated by immunoprecipitation and immunofluorescence, and binds to a specific epitope of this antigen presented in association with HLA-A2.1 molecules at the surface of these cells, as demonstrated by flow cytometry. Thus this MAb could be useful to better understand how this new protein presents in Ph-positive cells beside the canonical Bcr/Abl fusion proteins.
Characterization of a monoclonal antibody specific for novel Bcr/Abl out-of-frame fusion proteins / C. Casnici, G. Volpe, K. Crotta, C. Panuzzo, D. Lattuada, C.A. Cabras, R. Longhi, G. Saglio, O. Marelli. - In: HYBRIDOMA. - ISSN 0272-457X. - 30:3(2011), pp. 261-269.
|Titolo:||Characterization of a monoclonal antibody specific for novel Bcr/Abl out-of-frame fusion proteins|
CASNICI, CLAUDIA (Primo)
MARELLI, ORNELLA (Ultimo)
|Parole Chiave:||BCR-ABL ; out of frame ; monoclonal antibody|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Data di pubblicazione:||2011|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1089/hyb.2010.0122|
|Appare nelle tipologie:||01 - Articolo su periodico|