We report the isolation and characterisation of DKT1, a new carrot Kþ channel a-subunit belonging to the Shaker-like family. DKT1 is expressed in many tissues of the adult plant, suggesting that it may play important roles in both nutrition and other important physiological processes. During embryo development, DKT1 is expressed at later phases implying the involvement of Kþ in embryo maturation. When co-expressed with KDC1 in Xenopus oocytes, DKT1 is able to form functional, heteromeric channels, suggesting that possible interactions between these two ion channels in plant tissues may modulate Kþ uptake. 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

DKT1, a novel K+ channel from carrot, forms functional heteromeric channels with KDC1 / E. Formentina, S. Varotto, A. Costa, P. Downey, M. Bregante, A. Naso, C. Picco, F. Gambale, F. Lo Schiavo. - In: FEBS LETTERS. - ISSN 0014-5793. - 573:1-3(2004 Jul), pp. 61-67.

DKT1, a novel K+ channel from carrot, forms functional heteromeric channels with KDC1

A. Costa;
2004

Abstract

We report the isolation and characterisation of DKT1, a new carrot Kþ channel a-subunit belonging to the Shaker-like family. DKT1 is expressed in many tissues of the adult plant, suggesting that it may play important roles in both nutrition and other important physiological processes. During embryo development, DKT1 is expressed at later phases implying the involvement of Kþ in embryo maturation. When co-expressed with KDC1 in Xenopus oocytes, DKT1 is able to form functional, heteromeric channels, suggesting that possible interactions between these two ion channels in plant tissues may modulate Kþ uptake. 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Heteromeric channel; K+ channel; KDC1, K+ Daucus carota 1; RACE, rapid amplification of cDNA ends; RT-PCR, reverse transcriptase-polymerase chain reaction; S.D., standard deviation; S.E., standard error; SAM, shoot apical meristem
Settore BIO/10 - Biochimica
lug-2004
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/169651
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