D-Amino acid oxidase and D-aspartate oxidase are two well-known FAD-containing flavooxidases that catalyze the same reaction (the oxidative deamination) on different D-amino acids. D-aspartate oxidase is specific for acidic D-amino acids (i.e., D-aspartate and D-glutamate) and D-amino acid oxidase is active on neutral and polar D-amino acids (a low activity is also detected on basic D-amino acids). The assay of these flavoenzymes is of utmost importance in different fields because D-amino acids are common constituents of bacterial cell walls, are present in foods and because free D-serine and D-aspartic acid were identified in brain and peripheral tissues of mammals. In this chapter, we report on the most used methods employed to assay the activity of D-amino acid oxidase and D-aspartate oxidase. Interestingly, their activity can be followed using different assays, namely D-amino acid or oxygen consumption, α-keto acid or ammonia production, or using artificial dyes as final indicator of the flavin redox reaction.

Assays of D-amino acid oxidases / G. Tedeschi, L. Pollegioni, A. Negri (METHODS IN MOLECULAR BIOLOGY). - In: Unnatural amino acids : methods and protocols / [a cura di] L. Pollegioni, S. Servi. - New York : Humana Press, 2012. - ISBN 978-1-61779-330-1. - pp. 381-395

Assays of D-amino acid oxidases

G. Tedeschi
Primo
;
A. Negri
Ultimo
2012

Abstract

D-Amino acid oxidase and D-aspartate oxidase are two well-known FAD-containing flavooxidases that catalyze the same reaction (the oxidative deamination) on different D-amino acids. D-aspartate oxidase is specific for acidic D-amino acids (i.e., D-aspartate and D-glutamate) and D-amino acid oxidase is active on neutral and polar D-amino acids (a low activity is also detected on basic D-amino acids). The assay of these flavoenzymes is of utmost importance in different fields because D-amino acids are common constituents of bacterial cell walls, are present in foods and because free D-serine and D-aspartic acid were identified in brain and peripheral tissues of mammals. In this chapter, we report on the most used methods employed to assay the activity of D-amino acid oxidase and D-aspartate oxidase. Interestingly, their activity can be followed using different assays, namely D-amino acid or oxygen consumption, α-keto acid or ammonia production, or using artificial dyes as final indicator of the flavin redox reaction.
Analytical detection; d-Amino acid oxidase; d-Aspartate oxidase; Enzymatic assay
Settore BIO/10 - Biochimica
Book Part (author)
File in questo prodotto:
File Dimensione Formato  
Chapter 26.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 4.6 MB
Formato Adobe PDF
4.6 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/169484
Citazioni
  • ???jsp.display-item.citation.pmc??? 5
  • Scopus 11
  • ???jsp.display-item.citation.isi??? 36
social impact