Calculation of uncertainty of measurement of the catalytic activity concentration of alanine amminotransferase (ALT) in serum by the IFCC reference procedure. The goal of standardization of measurements in Laboratory Medicine is to achieve comparable results in human samples, independent of the laboratory and/or the method used. This can be achieved by the adoption of the “reference system” approach, based on the concept of metrological traceability and a hierarchy of measurement procedures. The reference system requires reference procedures, reference materials and reference laboratories, which are able to produce results within defined limits of uncertainty. The model used by the reference laboratories to estimate the measurement uncertainty is the “bottom-up” approach proposed by the Guide to the expression of uncertainty of measurement (GUM). The purpose of this study was to estimate the uncertainty associated with the measurement of the catalytic activity concentration of the enzyme ALT by the IFCC reference procedure using this approach. All the sources of uncertainty were identified and their contribution to the final expanded uncertainty was calculated using literature data or ad hoc experiments. We obtained an expanded uncertainty of 2.36%, comparable to that already calculated and published by other accredited reference laboratories. The uncertainty budget was built showing that concentrations and lot of reagents and measurement repeatability are the main components of the expanded uncertainty of the ALT measurements.
|Titolo:||Stima dell’incertezza della misura della concentrazione di attività catalitica dell’alanina amminotransferasi (ALT) nel siero mediante il metodo di riferimento IFCC|
PANTEGHINI, MAURO (Ultimo)
|Settore Scientifico Disciplinare:||Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica|
|Data di pubblicazione:||2011|
|Appare nelle tipologie:||01 - Articolo su periodico|