The use of platelet-enriched plasma has been proposed as a source of growth factors in tissue regeneration. The molecular mechanism at the basis of PRP action is not yet understood. The recent finding that PRP is a potent chemoattractive agent for osteoblasts, prompted us to investigate whether the increased cell migration might be due to cytoskeleton rearrangements. PRP pretreatment of SaOS-2 cells (osteoblastic cell line) increases the capacity of migration in a dose and time dependent manner. Interestingly, PRP pre-treatment induces cytoskeleton reorganization of the actin microfilaments, promoting the acquisition of a migratory phenotype in a time dose dependent manner. We also investigated the effects of a PRP pretreatment on microtubules and also in this case we observe an acquisition of a migratory phenotype. Adding an antibody against PDGF in PRP pretreatment the effect on migration capacity and actin remodelling was completely abolished. We observe also that a PRP pretreatment induces the induction of the mRNA espression of alpha chain of PDGF receptor in a short time, while for a long time the pretreatment inhibits the mRNA espression of the same chain. In conclusion we can say that the pretreatment with PRP is able to increase the migratory ability of osteoblastic cells by cytoskeleton reorganization. The molecular pathway linking PRP to microfilaments and microtubules rearrangement is probably mediated by PDGF signalling.

PRP e rimodellamento citoscheletrico : dagli effetti cellulari all'impiego clinico / L. Casati ; tutor: P. Castano, F. Celotti; coordinatore ; M. Gioia. DIPARTIMENTO DI MORFOLOGIA UMANA, DIPARTIMENTO DI ENDOCRINOLOGIA, FISIOPATOLOGIA E BIOLOGIA APPLICATA, 2010 Jan 12. 22. ciclo, Anno Accademico 2008/2009.

PRP e rimodellamento citoscheletrico : dagli effetti cellulari all'impiego clinico

L. Casati
2010

Abstract

The use of platelet-enriched plasma has been proposed as a source of growth factors in tissue regeneration. The molecular mechanism at the basis of PRP action is not yet understood. The recent finding that PRP is a potent chemoattractive agent for osteoblasts, prompted us to investigate whether the increased cell migration might be due to cytoskeleton rearrangements. PRP pretreatment of SaOS-2 cells (osteoblastic cell line) increases the capacity of migration in a dose and time dependent manner. Interestingly, PRP pre-treatment induces cytoskeleton reorganization of the actin microfilaments, promoting the acquisition of a migratory phenotype in a time dose dependent manner. We also investigated the effects of a PRP pretreatment on microtubules and also in this case we observe an acquisition of a migratory phenotype. Adding an antibody against PDGF in PRP pretreatment the effect on migration capacity and actin remodelling was completely abolished. We observe also that a PRP pretreatment induces the induction of the mRNA espression of alpha chain of PDGF receptor in a short time, while for a long time the pretreatment inhibits the mRNA espression of the same chain. In conclusion we can say that the pretreatment with PRP is able to increase the migratory ability of osteoblastic cells by cytoskeleton reorganization. The molecular pathway linking PRP to microfilaments and microtubules rearrangement is probably mediated by PDGF signalling.
12-gen-2010
Settore BIO/16 - Anatomia Umana
Settore BIO/17 - Istologia
CASTANO, PAOLO
CELOTTI, FABIO MARIA
GIOIA, MAGDA ENRICA
Doctoral Thesis
PRP e rimodellamento citoscheletrico : dagli effetti cellulari all'impiego clinico / L. Casati ; tutor: P. Castano, F. Celotti; coordinatore ; M. Gioia. DIPARTIMENTO DI MORFOLOGIA UMANA, DIPARTIMENTO DI ENDOCRINOLOGIA, FISIOPATOLOGIA E BIOLOGIA APPLICATA, 2010 Jan 12. 22. ciclo, Anno Accademico 2008/2009.
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/168519
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact