The liver sustains the greatest damage from ethanol (EtOH) abuse. EtOH and its metabolites impair hepatocyte metabolism, causing intracellular accumulation of proteins and lipids and increasing radical oxygen species production. These processes are toxic to the mitochondrial respiratory chain and to mitochondrial DNA. We have recently shown that supplementating the diet of rodents with an essential amino acid-enriched mixture (EAAem) significantly increases mitochondrial mass and number in cardiac and skeletal muscles and improves mitochondrial function in aged animals. Thus, in this study we sought to test whether EAAem supplementation could reduce EtOH-induced liver damage. Groups of adult male Wistar rats were fed a standard diet and water ad libitum (the control group), drinking water with 20 percent EtOH (the EtOH group), or drinking water with 20 percent EtOH and EAAem supplementation (1.5 g/kg/day) (the EtOH+EAAem group) for 2 months. The blood EtOH concentration was measured, and markers for fat (Oil-Red-O), mitochondria (Grp75, Cyt-c-ox), endoplasmic reticulum (Grp78), and inflammation (Heme Oxigenase 1, iNOS, and peroxisomes) were analyzed in the liver of animals in the various experimental groups. EAAem supplementation in EtOH-drinking rats ameliorated EtOH-induced changes in liver structure by limiting steatosis, recruiting more mitochondria and peroxisomes mainly to perivenous hepatocytes, stimulating or restoring antioxidant markers, limiting the expression of inflammatory processes, and reducing ER stress. Taken together, these results suggest that EAAem supplementation may represent a promising strategy to prevent and treat EtOH-induced liver damage
Essential amino acid supplementation decreases liver damage induced by chronic ethanol consumption in rats / G. Corsetti, A. Stacchiotti, L. Tedesco, G. D'Antona, E. Pasini, F.S. Dioguardi, E. Nisoli, R. Rezzani. - In: INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY. - ISSN 0394-6320. - 24:3(2011 Jul), pp. 611-619.
|Titolo:||Essential amino acid supplementation decreases liver damage induced by chronic ethanol consumption in rats|
|Parole Chiave:||dietary supplements; alcohol drinking; amino acids, essential; animals; azo compounds; body weight; catalase; central nervous system depressants; coloring agents; electron transport complex iv; ethanol; fatty liver; hepatitis, alcoholic; histocytochemistry; immunohistochemistry; inflammation; liver; male; organ size; rats; rats, wistar|
|Settore Scientifico Disciplinare:||Settore MED/09 - Medicina Interna|
|Data di pubblicazione:||lug-2011|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1177/039463201102400307|
|Appare nelle tipologie:||01 - Articolo su periodico|