PURPOSE: The authors present a protocol for the in vivo evaluation, using different imaging techniques, of lymph node (LN) homing of tumor-specific dendritic cells (DCs) in a murine breast cancer model. PROCEDURES: Bone marrow DCs were labeled with paramagnetic nanoparticles (MNPs) or (111)In-oxine. Antigen loading was performed using tumor lysate. Mature DCs were injected into the footpads of transgenic tumor-bearing mice (MMTV-Ras) and DC migration was tracked by magnetic resonance imaging (MRI) and single-photon emission computed tomography (SPECT). Ex vivo analyses were performed to validate the imaging data. RESULTS: DC labeling, both with MNPs and with (111)In-oxine, did not affect DC phenotype or functionality. MRI and SPECT allowed the detection of iron and (111)In in both axillary and popliteal LNs. Immunohistochemistry and γ-counting revealed the presence of DCs in LNs. CONCLUSIONS: MRI and SPECT imaging, by allowing in vivo dynamic monitoring of DC migration, could further the development and optimization of efficient anti-cancer vaccines.

In vivo imaging of lymph node migration of MNP- and (111) in-labeled dendritic cells in a transgenic mouse model of breast cancer (MMTV-Ras) / C. Martelli, M. Borelli, L. Ottobrini, V. Rainone, A. Degrassi, M. Russo, U. Gianelli, S. Bosari, C. Fiorini, D. Trabattoni, M. Clerici, G. Lucignani. - In: MOLECULAR IMAGING AND BIOLOGY. - ISSN 1536-1632. - 14:2(2012 Apr), pp. 183-196. [10.1007/s11307-011-0496-0]

In vivo imaging of lymph node migration of MNP- and (111) in-labeled dendritic cells in a transgenic mouse model of breast cancer (MMTV-Ras)

C. Martelli;M. Borelli;L. Ottobrini;V. Rainone;M. Russo;U. Gianelli;S. Bosari;D. Trabattoni;M. Clerici;G. Lucignani
2012-04

Abstract

PURPOSE: The authors present a protocol for the in vivo evaluation, using different imaging techniques, of lymph node (LN) homing of tumor-specific dendritic cells (DCs) in a murine breast cancer model. PROCEDURES: Bone marrow DCs were labeled with paramagnetic nanoparticles (MNPs) or (111)In-oxine. Antigen loading was performed using tumor lysate. Mature DCs were injected into the footpads of transgenic tumor-bearing mice (MMTV-Ras) and DC migration was tracked by magnetic resonance imaging (MRI) and single-photon emission computed tomography (SPECT). Ex vivo analyses were performed to validate the imaging data. RESULTS: DC labeling, both with MNPs and with (111)In-oxine, did not affect DC phenotype or functionality. MRI and SPECT allowed the detection of iron and (111)In in both axillary and popliteal LNs. Immunohistochemistry and γ-counting revealed the presence of DCs in LNs. CONCLUSIONS: MRI and SPECT imaging, by allowing in vivo dynamic monitoring of DC migration, could further the development and optimization of efficient anti-cancer vaccines.
dendritic cells ; MRI ; SPECT ; in vivo imaging ; vaccine protocol ; breast cancer
Settore MED/36 - Diagnostica per Immagini e Radioterapia
Settore MED/04 - Patologia Generale
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/167204
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