PURPOSE: The authors present a protocol for the in vivo evaluation, using different imaging techniques, of lymph node (LN) homing of tumor-specific dendritic cells (DCs) in a murine breast cancer model. PROCEDURES: Bone marrow DCs were labeled with paramagnetic nanoparticles (MNPs) or (111)In-oxine. Antigen loading was performed using tumor lysate. Mature DCs were injected into the footpads of transgenic tumor-bearing mice (MMTV-Ras) and DC migration was tracked by magnetic resonance imaging (MRI) and single-photon emission computed tomography (SPECT). Ex vivo analyses were performed to validate the imaging data. RESULTS: DC labeling, both with MNPs and with (111)In-oxine, did not affect DC phenotype or functionality. MRI and SPECT allowed the detection of iron and (111)In in both axillary and popliteal LNs. Immunohistochemistry and γ-counting revealed the presence of DCs in LNs. CONCLUSIONS: MRI and SPECT imaging, by allowing in vivo dynamic monitoring of DC migration, could further the development and optimization of efficient anti-cancer vaccines.
In vivo imaging of lymph node migration of MNP- and (111) in-labeled dendritic cells in a transgenic mouse model of breast cancer (MMTV-Ras) / C. Martelli, M. Borelli, L. Ottobrini, V. Rainone, A. Degrassi, M. Russo, U. Gianelli, S. Bosari, C. Fiorini, D. Trabattoni, M. Clerici, G. Lucignani. - In: MOLECULAR IMAGING AND BIOLOGY. - ISSN 1536-1632. - 14:2(2012 Apr), pp. 183-196.
|Titolo:||In vivo imaging of lymph node migration of MNP- and (111) in-labeled dendritic cells in a transgenic mouse model of breast cancer (MMTV-Ras)|
MARTELLI, CRISTINA (Primo)
BORELLI, MANUELA (Secondo)
CLERICI, MARIO SALVATORE (Penultimo)
LUCIGNANI, GIOVANNI (Ultimo)
|Parole Chiave:||dendritic cells ; MRI ; SPECT ; in vivo imaging ; vaccine protocol ; breast cancer|
|Settore Scientifico Disciplinare:||Settore MED/36 - Diagnostica per Immagini e Radioterapia|
Settore MED/04 - Patologia Generale
|Data di pubblicazione:||apr-2012|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1007/s11307-011-0496-0|
|Appare nelle tipologie:||01 - Articolo su periodico|