Some lysosomal glycohydrolases (N-acetyl-β-d-glucosaminidase and their major isoenzymes, β-d-glucuronidase, α-d-galactosidase, β-d-galactosidase and α-d-glucosidase) were investigated in the plasma of 36 preterm infants with respiratory distress, 11 of whom developed bronchopulmonary dysplasia (BPD), in order to evaluate the role of the lysosomal apparatus in the disease. Enzyme activity was assayed fluorimetrically; the major N-acetyl-β-d-glucosaminidase (NAG) isoenzymes were separated using a routine chromatofocusing procedure; the diagnostic efficiency was evaluated by Bayes theorem. The mean levels of almost all glycohydrolases considered were significantly higher in BPD than in non-BPD infants. Among NAG major isoenzymes, an increase was found only in form A. No variation was evident in the plasma levels of glycohydrolases during dexamethasone therapy. Data from a retrospective analysis performed in all preterms considered, show that α-d-galactosidase and β-d-galactosidase differentiate a posteriori BPD and non-BPD subjects. These enzymes, after a priori verification of their diagnostic potential in preterm infants at risk of BPD development, could acquire an important predictive value. Copyright (C) 1998 Elsevier Science B.V.

Lysosomal enzymes in preterm infants with bronchopulmonary dysplasia: a potential diagnostic marker / G. Goi, C. Bairati, L. Massaccesi, A. Lombardo, L. Bonafè, V. Zanardo, A. Burlina. - In: CLINICA CHIMICA ACTA. - ISSN 0009-8981. - 278:1(1998), pp. 23-34.

Lysosomal enzymes in preterm infants with bronchopulmonary dysplasia: a potential diagnostic marker

G. Goi
Primo
;
C. Bairati
Secondo
;
L. Massaccesi;A. Lombardo;
1998

Abstract

Some lysosomal glycohydrolases (N-acetyl-β-d-glucosaminidase and their major isoenzymes, β-d-glucuronidase, α-d-galactosidase, β-d-galactosidase and α-d-glucosidase) were investigated in the plasma of 36 preterm infants with respiratory distress, 11 of whom developed bronchopulmonary dysplasia (BPD), in order to evaluate the role of the lysosomal apparatus in the disease. Enzyme activity was assayed fluorimetrically; the major N-acetyl-β-d-glucosaminidase (NAG) isoenzymes were separated using a routine chromatofocusing procedure; the diagnostic efficiency was evaluated by Bayes theorem. The mean levels of almost all glycohydrolases considered were significantly higher in BPD than in non-BPD infants. Among NAG major isoenzymes, an increase was found only in form A. No variation was evident in the plasma levels of glycohydrolases during dexamethasone therapy. Data from a retrospective analysis performed in all preterms considered, show that α-d-galactosidase and β-d-galactosidase differentiate a posteriori BPD and non-BPD subjects. These enzymes, after a priori verification of their diagnostic potential in preterm infants at risk of BPD development, could acquire an important predictive value. Copyright (C) 1998 Elsevier Science B.V.
Bronchopulmonary dysplasia; Dexamethasone therapy; Diagnostic marker; Lysosomal enzymes; N-Acetyl-β-d-glucosaminidase isoenzymes; Preterm infants
Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica
Settore MED/46 - Scienze Tecniche di Medicina di Laboratorio
1998
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/166443
Citazioni
  • ???jsp.display-item.citation.pmc??? 2
  • Scopus 6
  • ???jsp.display-item.citation.isi??? 5
social impact