Aims Bone marrow mesenchymal stromal cell (BMStC) transplantation into the infarcted heart improves left ventricular function and cardiac remodelling. However, it has been suggested that tissue-specific cells may be better for cardiac repair than cells from other sources. The objective of the present work has been the comparison of in vitro and in vivo properties of adult human cardiac stromal cells (CStC) to those of syngeneic BMStC.Methods and resultsAlthough CStC and BMStC exhibited a similar immunophenotype, their gene, microRNA, and protein expression profiles were remarkably different. Biologically, CStC, compared with BMStC, were less competent in acquiring the adipogenic and osteogenic phenotype but more efficiently expressed cardiovascular markers. When injected into the heart, in rat a model of chronic myocardial infarction, CStC persisted longer within the tissue, migrated into the scar, and differentiated into adult cardiomyocytes better than BMStC.ConclusionOur findings demonstrate that although CStC and BMStC share a common stromal phenotype, CStC present cardiovascular-associated features and may represent an important cell source for more efficient cardiac repair.
Human cardiac and bone marrow stromal cells exhibit distinctive properties related to their origin / A. Rossini, C. Frati, C. Lagrasta, G. Graiani, A. Scopece, S. Cavalli, E. Musso, M. Baccarin, M. Di Segni, F. Fagnoni, A. Germani, E. Quaini, M. Mayr, Q. Xu, A.F. Barbuti, D. DiFrancesco, G. Pompilio, F. Quaini, C. Gaetano, M.C. Capogrossi. - In: CARDIOVASCULAR RESEARCH. - ISSN 0008-6363. - 89:3(2011 Feb 15), pp. 650-660.
|Titolo:||Human cardiac and bone marrow stromal cells exhibit distinctive properties related to their origin|
ROSSINI, ALESSANDRA (Primo)
|Parole Chiave:||Cardiac and bone marrow stromal cells; Cardiovascular differentiation; Cardiac regeneration; Myocardial infarction|
|Settore Scientifico Disciplinare:||Settore BIO/09 - Fisiologia|
Settore MED/11 - Malattie dell'Apparato Cardiovascolare
|Data di pubblicazione:||15-feb-2011|
|Data ahead of print / Data di stampa:||10-set-2010|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1093/cvr/cvq290|
|Appare nelle tipologie:||01 - Articolo su periodico|
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