The human antiapoptotic bcl-2 gene has been discovered in t(14;18) B-cell leukemias/lymphomas because of its overexpression caused at a transcriptional control level by the bcl-2/IgH fusion gene. We were the first to disclose the post-transcriptional control of bcl-2 expression mediated by interactions of an adenine + uracil (AU)-rich element (ARE) in the 3'-UTR of bcl-2 mRNA with AU-binding proteins (AUBPs). Here, we identify and characterize zeta-crystallin as a new bcl-2 AUBP, whose silencing or overexpression has impact on bcl-2 mRNA stability. An increased Bcl-2 level observed in normal phytohemagglutinin (PHA)-activated T lymphocytes, acute lymphatic leukemia (ALL) T-cell lines, and T cells of patients with leukemia in comparison with normal non-PHA-activated T lymphocytes was concomitant with an increase in zeta-crystallin level. The specific association of zeta-crystallin with the bcl-2 ARE was significantly enhanced in T cells of patients with ALL, which accounts for the higher stability of bcl-2 mRNA and suggests a possible contribution of zeta-crystallin to bcl-2 overexpression occurring in this leukemia.
zeta-Crystallin is a bcl-2 mRNA binding protein involved in bcl-2 overexpression in T-cell acute lymphocytic leukemia / A. Lapucci, M. Lulli, A. Amedei, L. Papucci, E. Witort, F. Di Gesualdo, F. Bertolini, G. Brewer, A.N. Nicolin, A.T. Bevilacqua, N. Schiavone, D. Morello, M. Donnini, S. Capaccioli. - In: THE FASEB JOURNAL. - ISSN 0892-6638. - 24:6(2010), pp. 1852-1865.
|Titolo:||zeta-Crystallin is a bcl-2 mRNA binding protein involved in bcl-2 overexpression in T-cell acute lymphocytic leukemia|
|Parole Chiave:||post-transcriptional control ; cancer ; ARE-binding protein|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
Settore BIO/11 - Biologia Molecolare
Settore BIO/13 - Biologia Applicata
|Data di pubblicazione:||2010|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1096/fj.09-140459|
|Appare nelle tipologie:||01 - Articolo su periodico|