Genetic diversity of 'bois noir' phytoplasma populations in two vineyards of Verona province (Veneto region, North-Eastern Italy) was investigated by the use of multilocus analysis of gene sequences amplified in the polymerase chain reaction. Based on results from restriction fragment length polymorphism profiling and nucleotide sequence alignments of 16S rRNA, tuf, hlyC, trxAtruB, cbiQ-glyA, and rplS-csdB genes, 'bois noir' phytoplasma strains were grouped in five SNP lineages. The data suggest that strain composition of BN phytoplasma populations may be modified by ecological relationships in vineyards.
Molecular characterization of ‘bois noir’ phytoplasma populations from North-Eastern Italy / F. QUAGLINO, N. MORI, Y. ZHAO, G. ZANINI, P. CASATI, D. BULGARI, A. ZORLONI, R.E. DAVIS, P.A. BIANCO. - In: BULLETIN OF INSECTOLOGY. - ISSN 1721-8861. - 64:SUPPLEMENT(2011), pp. S231-S232. (Intervento presentato al 2. convegno International Phytoplasmologist Working Group Meeting (IPWG) tenutosi a Neustadt/Weinstrasse (Germany) nel 2011).
Molecular characterization of ‘bois noir’ phytoplasma populations from North-Eastern Italy
F. QuaglinoPrimo
;P. Casati;D. Bulgari;A. Zorloni;P.A. BiancoUltimo
2011
Abstract
Genetic diversity of 'bois noir' phytoplasma populations in two vineyards of Verona province (Veneto region, North-Eastern Italy) was investigated by the use of multilocus analysis of gene sequences amplified in the polymerase chain reaction. Based on results from restriction fragment length polymorphism profiling and nucleotide sequence alignments of 16S rRNA, tuf, hlyC, trxAtruB, cbiQ-glyA, and rplS-csdB genes, 'bois noir' phytoplasma strains were grouped in five SNP lineages. The data suggest that strain composition of BN phytoplasma populations may be modified by ecological relationships in vineyards.
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