The efficient production of recombinant proteins in Escherichia coli requires a proper termination of translation to ensure the synthesis of only the desired product. During the recombinant production of Bacillus subtilis flgM in E. coli, we detected an additional polypeptide of molecular mass higher than the expected, corresponding to a product of a translational readthrough of the UGA stop codon. In this paper we show that the readthrough was abolished when the synthesis of the recombinant protein was carried out at 25°C. The possible causes that contribute to reduce the proportion of readthrough protein species against the correct terminated product are discussed.
|Titolo:||Growth at low temperature suppresses readthrough of the UGA stop codon during the expression of Bacillus subtilis flgM gene in Escherichia coli|
CECILIANI, FABRIZIO (Secondo)
|Settore Scientifico Disciplinare:||Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria|
|Data di pubblicazione:||2003|
|Digital Object Identifier (DOI):||10.1016/S0168-1656(02)00340-1|
|Appare nelle tipologie:||01 - Articolo su periodico|