The application of biocides is a traditional method of controlling biodecay of outdoor cultural heritage. Chlorophyll degradation to phaeopigments is used to test the biocidal efficacy of the antimicrobial agents. In the present study, the usefulness of color measurements in estimating chlorophyll degradation was investigated. An aeroterrestrial stone biofilm-forming cyanobacterium of the genus Nostoc was chosen as test organism, comparing its different behaviour in both planktonic and biofilm mode of growth against the isothiazoline biocide Biotin T(A (R)). Changes in A(435 nm)/A(415 nm) and A(665 nm)/A(665a nm) and in the chlorophyll a and adenosine triphosphate (ATP) cell content were compared with the variations in the CIELAB color parameters (L*, a*, b*, C*(ab) and h(ab)). Our findings showed that both the phaeophytination indexes are useful in describing degradation of chlorophyl a to phaeopigments. Moreover, the CIELAB color parameters represented an effective tool in describing chlorophyll degradation. L* CIELAB parameter appeared to be the most informative parameter in describing the biocidal activity of Biotin T(A (R)) against Nostoc sp. in both planktonic and biofilm mode of growth.

Color measurements as a reliable method for estimating chlorophyll degradation to phaeopigments / P. Sanmartín, F. Villa, B. Silva, F. Cappitelli, B. Prieto. - In: BIODEGRADATION. - ISSN 0923-9820. - 22:4(2011), pp. 763-771.

Color measurements as a reliable method for estimating chlorophyll degradation to phaeopigments

F. Villa
Secondo
;
F. Cappitelli
Penultimo
;
2011

Abstract

The application of biocides is a traditional method of controlling biodecay of outdoor cultural heritage. Chlorophyll degradation to phaeopigments is used to test the biocidal efficacy of the antimicrobial agents. In the present study, the usefulness of color measurements in estimating chlorophyll degradation was investigated. An aeroterrestrial stone biofilm-forming cyanobacterium of the genus Nostoc was chosen as test organism, comparing its different behaviour in both planktonic and biofilm mode of growth against the isothiazoline biocide Biotin T(A (R)). Changes in A(435 nm)/A(415 nm) and A(665 nm)/A(665a nm) and in the chlorophyll a and adenosine triphosphate (ATP) cell content were compared with the variations in the CIELAB color parameters (L*, a*, b*, C*(ab) and h(ab)). Our findings showed that both the phaeophytination indexes are useful in describing degradation of chlorophyl a to phaeopigments. Moreover, the CIELAB color parameters represented an effective tool in describing chlorophyll degradation. L* CIELAB parameter appeared to be the most informative parameter in describing the biocidal activity of Biotin T(A (R)) against Nostoc sp. in both planktonic and biofilm mode of growth.
Chlorophyll; Phaeophytination; Non-destructive methods; CIELAB color measurements; Biocide
Settore AGR/16 - Microbiologia Agraria
2011
Article (author)
File in questo prodotto:
File Dimensione Formato  
Sanmartın2011-phaeopigments-BIODEGRADATION.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 273.4 kB
Formato Adobe PDF
273.4 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/159025
Citazioni
  • ???jsp.display-item.citation.pmc??? 6
  • Scopus 52
  • ???jsp.display-item.citation.isi??? 46
social impact