Recent evidence points to a strong relationship between increased mitochondrial biogenesis and increased survival in eukaryotes. Branched-chain amino acids (BCAAs) have been shown to extend chronological life span in yeast. However, the role of these amino acids in mitochondrial biogenesis and longevity in mammals is unknown. Here, we show that a BCAA-enriched mixture (BCAAem) increased the average life span of mice. BCAAem supplementation increased mitochondrial biogenesis and sirtuin 1 expression in primary cardiac and skeletal myocytes and in cardiac and skeletal muscle, but not in adipose tissue and liver of middle-aged mice, and this was accompanied by enhanced physical endurance. Moreover, the reactive oxygen species (ROS) defense system genes were upregulated, and ROS production was reduced by BCAAem supplementation. All of the BCAAem-mediated effects were strongly attenuated in endothelial nitric oxide synthase null mutant mice. These data reveal an important antiaging role of BCAAs mediated by mitochondrial biogenesis in mammals.
Branched-chain amino acid supplementation promotes survival and supports cardiac and skeletal muscle mitochondrial biogenesis in middle-aged mice / G. D'Antona, M. Ragni, A. Cardile, L.E.M. Tedesco, M. Dossena, F. Bruttini, F. Caliaro, G. Corsetti, R. Bottinelli, M. Carruba, A. Valerio, E. Nisoli. - In: CELL METABOLISM. - ISSN 1550-4131. - 12:4(2010), pp. 362-372.
|Titolo:||Branched-chain amino acid supplementation promotes survival and supports cardiac and skeletal muscle mitochondrial biogenesis in middle-aged mice|
RAGNI, MAURIZIO (Secondo)
NISOLI, ENZO (Ultimo)
|Parole Chiave:||Mitochondrial biogenesis ; life span ; nitric oxide ; endothelial nitric oxide synthase ; mammalian target of rapamycin ; reactive oxygen species ; white adipose tissue ; brown adipose tissue ; skeletal muscle ; cardiac muscle|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Data di pubblicazione:||2010|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.cmet.2010.08.016|
|Appare nelle tipologie:||01 - Articolo su periodico|