In vitro release modulation and conformational stabilization of a model protein using swellable polyamidoamine nanosponges of b-cyclodextrin

Swaminathan, S.; Cavalli, R.; Trotta, F.; Ferruti, P.; Ranucci, E.; Gerges, I.; Manfredi, A.G.; Marinotto, D.; Vavia, P.R.

doi:10.1007/s10847-010-9765-9

IRIS Institutional Research Information System - AIR Archivio Istituzionale della Ricerca

IRIS è il sistema di gestione integrata dei dati della ricerca (persone, progetti, pubblicazioni, attività) adottato dall'Università degli Studi di Milano.

AIR nasce in UNIMI nel 2006 con lo scopo di raccogliere, documentare e conservare le informazioni sulla produzione scientifica dell'Università degli Studi di Milano e costituisce di fatto l'Anagrafe della ricerca dell'Ateneo.

New swellable cyclodextrin-based poly(amidoamine) nanosponges, named PAA-NS10 and PAA-NS11, were synthesized by crosslinking β-cyclodextrin with either 2,2-bisacrylamidoacetic acid or with polyamidoamine segments deriving from 2,2-bisacrylamidoacetic acid and 2-methylpiperazine, respectively. Water uptake studies showed a tremendous swelling capacity of both nanosponges, forming hydrogels. Time dependent swelling experiments in various aqueous media showed that the nanosponge hydrogels were stable over a period of at least 72 h maintaining their integrity. Thermal analysis showed that the two nanosponges were stable up to 250 and 300 °C, respectively. Both PAA-NS10 and PAA-NS11 were converted to aqueous nanosuspensions using the High Pressure Homogenization technique. Bovine serum albumin (BSA) was used as model protein to study the encapsulating capacity of these new β-cyclodextrin-based PAA-nanosponges. High protein complexation capacity was observed, as confirmed by UV spectroscopy. BSA encapsulation efficiency was greater than 90% on w/w basis for both nanosponges. In vitro BSA release studies were carried out showing a prolonged release of albumin from the two swollen BSA loaded β-CD PAA-NS over a period of 24 h.

In vitro release modulation and conformational stabilization of a model protein using swellable polyamidoamine nanosponges of b-cyclodextrin / S. Swaminathan, R. Cavalli, F. Trotta, P. Ferruti, E. Ranucci, I. Gerges, A.G. Manfredi, D. Marinotto, P.R. Vavia. - In: JOURNAL OF INCLUSION PHENOMENA AND MACROCYCLIC CHEMISTRY. - ISSN 1388-3127. - 68:1/2(2010), pp. 183-191.

Titolo:	In vitro release modulation and conformational stabilization of a model protein using swellable polyamidoamine nanosponges of b-cyclodextrin
Autori:	S. Swaminathan R. Cavalli F. Trotta FERRUTI, PAOLO RANUCCI, ELISABETTA GERGES, IRINI MANFREDI, AMEDEA GIUSEPPINA MARINOTTO, DANIELE P. R. Vavia mostra contributor esterni nascondi contributor esterni
Parole Chiave:	Bovine serum albumin; Cyclodextrin; Poly(amidoamine) nanosponges; Prolonged release; Swellable nanosponges; Water uptake
Settore Scientifico Disciplinare:	Settore CHIM/04 - Chimica Industriale Settore CHIM/09 - Farmaceutico Tecnologico Applicativo
Data di pubblicazione:	2010
Rivista:	JOURNAL OF INCLUSION PHENOMENA AND MACROCYCLIC CHEMISTRY
Tipologia:	Article (author)
Digital Object Identifier (DOI):	http://dx.doi.org/10.1007/s10847-010-9765-9
Appare nelle tipologie:	01 - Articolo su periodico

File in questo prodotto:

Non ci sono file associati a questo prodotto.

Utilizza questo identificativo per citare o creare un link a questo documento:
http://hdl.handle.net/2434/154704

Citazioni

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.