The Mediterranean fruit fly Ceratitis capitata (Diptera: Tephritidae) is one of the most destructive agricultural pests, a polyphagus insect of relevant economic importance and is widespread in many regions around the world. It is the best-studied fruit fly pest at genetic and molecular level and much has been learned on its ecology and behaviour. An α-l-fucosidase has been recently hypothesized to be involved in sperm–egg interactions in Drosophila melanogaster and in other Drosophila species. Here, a complete cDNA encoding a putative α-l-fucosidase of the medfly was amplified using the reverse polymerase chain reaction (RT-PCR) with degenerate based on the conserved coding sequence information of several insect α-l-fucosidases, cloned and sequenced (GenBank accession no. FJ177429). The coding region consisted of 1482 bp which encoded a 485-residues protein (named CcFUCA) with a predicted molecular mass of 56.1 kDa. The deduced protein sequence showed 75% amino acid identity to D. melanogaster α-l-fucosidase, and in fact the phylogenetic tree analysis revealed that CcFUCA had closer relationships with the α-l-fucosidases of drosophilid species. The tissue expression analysis indicated that CcFuca was expressed in a single transcript in all tissues, suggesting a ubiquitous localization pattern of the encoded protein. Our findings provide novel insights on a gene encoding a protein potentially involved in primary gamete interactions in C. capitata.
Cloning, sequence identification and expression profile analysis of α-l-fucosidase gene from the Mediterranean fruit fly Ceratitis capitata / J. Intra, M.E. Perotti, M.E. Pasini. - In: JOURNAL OF INSECT PHYSIOLOGY. - ISSN 0022-1910. - 57:4(2011), pp. 452-461.
|Titolo:||Cloning, sequence identification and expression profile analysis of α-l-fucosidase gene from the Mediterranean fruit fly Ceratitis capitata|
|Parole Chiave:||Fertilization; FISH; Fucose; Gamete; In situ hybridization; Medfly; Phylogeny|
|Settore Scientifico Disciplinare:||Settore BIO/06 - Anatomia Comparata e Citologia|
|Data di pubblicazione:||2011|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.jinsphys.2011.01.007|
|Appare nelle tipologie:||01 - Articolo su periodico|