Extracellular nucleotides regulate many cellular functions through activation of purinergic receptors in the plasma membrane. Here, we show that in hematopoietic stem cell (HSC), ATP is stored in vesicles and released in a calcium-sensitive manner. HSC expresses ATP responsive P2X receptors and in vitro pharmacological P2X antagonism restrained hematopoietic progenitors proliferation, but not myeloid differentiation. In mice suffering from chronic inflammation, HSCs were significantly expanded and their cycling activity was sensitive to treatment with the P2X antagonist periodate-oxidized 2,3-dialdehyde ATP. Our results indicate that ATP acts as an autocrine stimulus in regulating HSCs pool size.

Cell-autonomous regulation of hematopoietic stem cell cycling activity by ATP / A. Casati, M. Frascoli, E. Traggiai, M. Proietti, U. Schenk, F.M. Grassi. - In: CELL DEATH AND DIFFERENTIATION. - ISSN 1350-9047. - 18:3(2011), pp. 396-404.

Cell-autonomous regulation of hematopoietic stem cell cycling activity by ATP

A. Casati
Primo
;
M. Proietti;U. Schenk
Penultimo
;
F.M. Grassi
Ultimo
2011

Abstract

Extracellular nucleotides regulate many cellular functions through activation of purinergic receptors in the plasma membrane. Here, we show that in hematopoietic stem cell (HSC), ATP is stored in vesicles and released in a calcium-sensitive manner. HSC expresses ATP responsive P2X receptors and in vitro pharmacological P2X antagonism restrained hematopoietic progenitors proliferation, but not myeloid differentiation. In mice suffering from chronic inflammation, HSCs were significantly expanded and their cycling activity was sensitive to treatment with the P2X antagonist periodate-oxidized 2,3-dialdehyde ATP. Our results indicate that ATP acts as an autocrine stimulus in regulating HSCs pool size.
Settore BIO/13 - Biologia Applicata
Settore BIO/14 - Farmacologia
2011
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/153815
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