We investigated levels of DNA damage in blood cells of barn swallows (Hirundo rustica) inhabiting the Chernobyl region to evaluate whether chronic exposure to low-level radioactive contamination continues to induce genetic damage in free-living populations of animals. Blood samples were obtained from barn swallows collected at sites with different background levels of radiation, including a relatively uncontaminated area. The extent of DNA damage was evaluated using the alkaline (pH = 12.1) comet assay, a robust and sensitive electrophoresis-based technique widely employed in research ranging from biomonitoring to clinical studies. We found that levels of DNA damage, as indexed by the extent of DNA migration, were increased in barn swallows living in areas surrounding Chernobyl when compared to swallows sampled at low-level sites. The results we obtained are consistent with previous findings on this same species, which showed that swallows breeding in areas heavily contaminated with radionuclides have increased mutation rates, higher oxidative stress and incidence of morphological aberrations and tumors. Overall, these results indicate that chronic exposure to radioactive contaminants, even 20 years after the accident at the Chernobyl nuclear power plant, continues to induce DNA damage in cells of free-living animals.
DNA damage in barn swallows (Hirundo rustica) from the Chernobyl region detected by use of the comet assay / A. Bonisoli-Alquati, A. Voris, T.A. Mousseau, A.P. Møller, N. Saino, M.D. Wyatt. - In: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. C. TOXICOLOGY & PHARMACOLOGY. - ISSN 1532-0456. - 151:3(2010), pp. 271-277.
|Titolo:||DNA damage in barn swallows (Hirundo rustica) from the Chernobyl region detected by use of the comet assay|
BONISOLI ALQUATI, ANDREA (Primo)
SAINO, NICOLA MICHELE FRANCESCO (Penultimo)
|Parole Chiave:||Barn swallow ; Chernobyl ; Comet assay ; Genetic damage ; Hirundo rustica|
|Settore Scientifico Disciplinare:||Settore BIO/07 - Ecologia|
Settore BIO/05 - Zoologia
|Data di pubblicazione:||2010|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.cbpc.2009.11.006|
|Appare nelle tipologie:||01 - Articolo su periodico|