BACKGROUND: Treatment of HIV-infected patients with interleukin-2 (IL-2) produces significant increases in CD4 T cell counts; however an associated qualitative improvement in cells function has yet to be conclusively demonstrated. By measuring mycobacterial killing activity, we evaluated IL-2-mediated functional immune enhancement ex vivo in immunological non-responders (INRs). METHODS AND FINDINGS: PBMC from 12 immunological non-responders (INRs) (CD4+<200/µl, HIV-RNA<50 cp/ml) on combination antiretroviral treatment (cART) were collected at baseline, and after 3 IL-2 cycles. Eight INRs receiving only cART were studied as controls. After 21 days of PBMC incubation with a virulent M. avium suspension, counts of residual colony forming units (CFUs) and concentrations of TNF-α, IL-10 and IFN-γ were determined. In IL-2 treated patients, a significant reduction in mean residual CFUs of PBMC cultures was observed (p<0.01). Moreover, following IL-2 treatment, significant increases in PBMC's IFNγ production (p = 0.02) and substantial reductions in IL-10 levels were observed. CONCLUSIONS: IL-2 therapy restores the ability of the lympho-monocyte system in eliciting an effective response against mycobacterial infections. Our data indicate the possibility of a clinical role held by IL-2 in enhancing the immune function of subjects unable to achieve immune competence through cART alone.
Qualitative immune modulation by Interleukin-2 (IL-2) adjuvant therapy in immunological non responder HIV-infected patients / F. Sabbatini, A. Bandera, G. Ferrario, D.L. Trabattoni, G.C. Marchetti, F. Franzetti, M. Clerici, A. Gori. - In: PLOS ONE. - ISSN 1932-6203. - 5:11(2010 Nov), pp. e14119.e14119-e14119.e14119.
|Titolo:||Qualitative immune modulation by Interleukin-2 (IL-2) adjuvant therapy in immunological non responder HIV-infected patients|
BANDERA, ALESSANDRA (Secondo)
CLERICI, MARIO SALVATORE (Penultimo)
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
Settore MED/17 - Malattie Infettive
|Data di pubblicazione:||nov-2010|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1371/journal.pone.0014119|
|Appare nelle tipologie:||01 - Articolo su periodico|