OBJECTIVE: We investigated mitochondrial DNA (mtDNA) content in the maternal circulation of normal pregnancies of different gestational ages and in pregnancies complicated by intrauterine growth restriction (IUGR). STUDY DESIGN: We examined 70 maternal blood samples: 13 nonpregnant women; 45 normal pregnancies, divided into the 3 trimesters; and 12 pregnancies complicated by IUGR. MtDNA content was determined by real-time quantitative polymerase chain reaction, using a genomic control and a target gene. RESULTS: A highly significant progressive reduction in circulating mtDNA was observed in pregnant women of first, second, and third trimesters and compared to nonpregnant women (mean value: 237, 188, 144, and 283, respectively; P .001). Moreover, mtDNA was significantly increased in women carrying IUGR fetuses compared to women with normal pregnancies (430 vs 144; P .001). CONCLUSION: MtDNA could provide new insight into the mechanisms that occur during physiological gestation. Furthermore, mtDNA content may help recognize the IUGR disease in pregnancy.

Maternal blood mitochondrial DNA content during normal and intrauterine growth restricted (IUGR) pregnancy / F. Colleoni, D. Lattuada, A. Garretto, M. Massari, C. Mandò, E. Somigliana, I. Cetin. - In: AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY. - ISSN 0002-9378. - 203:4(2010), pp. 365.e1-365.e6. [10.1016/j.ajog.2010.05.027]

Maternal blood mitochondrial DNA content during normal and intrauterine growth restricted (IUGR) pregnancy

F. Colleoni
Primo
;
D. Lattuada
Secondo
;
A. Garretto;M. Massari;C. Mandò;E. Somigliana
Penultimo
;
I. Cetin
Ultimo
2010

Abstract

OBJECTIVE: We investigated mitochondrial DNA (mtDNA) content in the maternal circulation of normal pregnancies of different gestational ages and in pregnancies complicated by intrauterine growth restriction (IUGR). STUDY DESIGN: We examined 70 maternal blood samples: 13 nonpregnant women; 45 normal pregnancies, divided into the 3 trimesters; and 12 pregnancies complicated by IUGR. MtDNA content was determined by real-time quantitative polymerase chain reaction, using a genomic control and a target gene. RESULTS: A highly significant progressive reduction in circulating mtDNA was observed in pregnant women of first, second, and third trimesters and compared to nonpregnant women (mean value: 237, 188, 144, and 283, respectively; P .001). Moreover, mtDNA was significantly increased in women carrying IUGR fetuses compared to women with normal pregnancies (430 vs 144; P .001). CONCLUSION: MtDNA could provide new insight into the mechanisms that occur during physiological gestation. Furthermore, mtDNA content may help recognize the IUGR disease in pregnancy.
Intrauterine growth restriction ; maternal blood ; mitochondrial DNA ; pregnancy :
Settore MED/40 - Ginecologia e Ostetricia
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/152157
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