Oocyte aspiration from equine follicles gives a low recovery rate and yields oocytes largely denuded of cumulus cells. Follicle scraping is labour intensive and increases the time required for collection, extending the holding time of oocytes that delays their maturation. The aim of this work was to develop an effective method for collecting equine oocytes combining the feature of aspiration (fastness) with that of scraping (high recovery rate of cumulus-intact oocytes). Furthermore, we examined differences in cumulus morphology and maturation rates, comparing this technique to aspiration and scraping, with or without tunica albuginea removal. Collection by vacuum pump aspiration was performed using a 16 g needle while the combination of aspiration and scraping was performed using a Tuohy needle (16 g) that is usually employed for inserting an epidural catheter and its tip shape is similar to a small curette. In unpeeled ovaries, the recovery rates by the Tuohy needle was higher (p < 0.05) than in the 16 g needle aspiration and in the scraped ovaries (57% vs. 36% and 47%) while the rate of cumulus-intact oocytes was higher than aspiration (46.9% vs. 39.36%) but lower than scraping (46.97%) (p < 0.001). In unpeeled ovaries there was no difference in maturation rate of oocytes recovered by Tuohy needle in respect to scraping in peeled ovaries (58.54% vs. 58.24% respectively; p < 0.05). In conclusion, combination of aspiration and scraping by Tuohy needle allows a faster and reliable collection of oocytes suitable for horse IVM.

Efficacy of tuohy needle in oocytes collection from excised mare ovaries / F. Cremonesi, K. Anderson, A.B. Lovati, A. Lange Consiglio. - In: REPRODUCTION IN DOMESTIC ANIMALS. - ISSN 0936-6768. - 45:suppl. 3(2010 Sep), pp. 83-83. ((Intervento presentato al 14. convegno Annual Conference of the European Society for Domestic Animal Reproduction tenutosi a Eger nel 2010.

Efficacy of tuohy needle in oocytes collection from excised mare ovaries

F. Cremonesi
Primo
;
A.B. Lovati
Penultimo
;
A. Lange Consiglio
Ultimo
2010-09

Abstract

Oocyte aspiration from equine follicles gives a low recovery rate and yields oocytes largely denuded of cumulus cells. Follicle scraping is labour intensive and increases the time required for collection, extending the holding time of oocytes that delays their maturation. The aim of this work was to develop an effective method for collecting equine oocytes combining the feature of aspiration (fastness) with that of scraping (high recovery rate of cumulus-intact oocytes). Furthermore, we examined differences in cumulus morphology and maturation rates, comparing this technique to aspiration and scraping, with or without tunica albuginea removal. Collection by vacuum pump aspiration was performed using a 16 g needle while the combination of aspiration and scraping was performed using a Tuohy needle (16 g) that is usually employed for inserting an epidural catheter and its tip shape is similar to a small curette. In unpeeled ovaries, the recovery rates by the Tuohy needle was higher (p < 0.05) than in the 16 g needle aspiration and in the scraped ovaries (57% vs. 36% and 47%) while the rate of cumulus-intact oocytes was higher than aspiration (46.9% vs. 39.36%) but lower than scraping (46.97%) (p < 0.001). In unpeeled ovaries there was no difference in maturation rate of oocytes recovered by Tuohy needle in respect to scraping in peeled ovaries (58.54% vs. 58.24% respectively; p < 0.05). In conclusion, combination of aspiration and scraping by Tuohy needle allows a faster and reliable collection of oocytes suitable for horse IVM.
Settore VET/10 - Clinica Ostetrica e Ginecologia Veterinaria
European Society for Domestic Animal Reproduction (ESDAR)
Article (author)
File in questo prodotto:
File Dimensione Formato  
2010-Reproduction_in_Domestic_Animals.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 727.8 kB
Formato Adobe PDF
727.8 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/151908
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? 0
social impact