Kcv from the chlorella virus PBCV-1 is a viral protein that forms a tetrameric, functional K+ channel in heterologous systems. Kcv can serve as a model system to study and manipulate basic properties of the K+ channel pore because its minimalistic structure (94 amino acids) produces basic features of ion channels, such as selectivity, gating, and sensitivity to blockers. We present a characterization of Kcv properties at the single-channel level. In symmetric 100 mM K+, single-channel conductance is 114 ± 11 pS. Two different voltage-dependent mechanisms are responsible for the gating of Kcv. “Fast” gating, analyzed by  distributions, is responsible for the negative slope conductance in the single-channel current–voltage curve at extreme potentials, like in MaxiK potassium channels, and can be explained by depletion-aggravated instability of the filter region. The presence of a “slow” gating is revealed by the very low (in the order of 1–4%) mean open probability that is voltage dependent and underlies the time-dependent component of the macroscopic current.

ANALYSIS OF INHERENT PROPERTIES OF ION CHANNELS FROM PBCV-1 CHLORELLA VIRUS AND INFLUENZA A VIRUS / M.l. Di Francesco ; tutor: Anna Moroni. DIPARTIMENTO DI BIOLOGIA, 2011 Jan 13. 23. ciclo, Anno Accademico 2010. [10.13130/di-francesco-mattia-lorenzo_phd2011-01-13].

ANALYSIS OF INHERENT PROPERTIES OF ION CHANNELS FROM PBCV-1 CHLORELLA VIRUS AND INFLUENZA A VIRUS.

M.L. DI FRANCESCO
2011

Abstract

Kcv from the chlorella virus PBCV-1 is a viral protein that forms a tetrameric, functional K+ channel in heterologous systems. Kcv can serve as a model system to study and manipulate basic properties of the K+ channel pore because its minimalistic structure (94 amino acids) produces basic features of ion channels, such as selectivity, gating, and sensitivity to blockers. We present a characterization of Kcv properties at the single-channel level. In symmetric 100 mM K+, single-channel conductance is 114 ± 11 pS. Two different voltage-dependent mechanisms are responsible for the gating of Kcv. “Fast” gating, analyzed by  distributions, is responsible for the negative slope conductance in the single-channel current–voltage curve at extreme potentials, like in MaxiK potassium channels, and can be explained by depletion-aggravated instability of the filter region. The presence of a “slow” gating is revealed by the very low (in the order of 1–4%) mean open probability that is voltage dependent and underlies the time-dependent component of the macroscopic current.
13-gen-2011
Settore BIO/04 - Fisiologia Vegetale
PBCV1-Kcv ; Chlorella virus
MORONI, ANNA
Doctoral Thesis
ANALYSIS OF INHERENT PROPERTIES OF ION CHANNELS FROM PBCV-1 CHLORELLA VIRUS AND INFLUENZA A VIRUS / M.l. Di Francesco ; tutor: Anna Moroni. DIPARTIMENTO DI BIOLOGIA, 2011 Jan 13. 23. ciclo, Anno Accademico 2010. [10.13130/di-francesco-mattia-lorenzo_phd2011-01-13].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/150556
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