A high-performance liquid chromatographic method for the determination of linezolid in human plasma was developed and validated. After precipitation of plasma proteins with perchloric acid, the protein-free supernatant was separated by isocratic reverse-phase chromatography on a X Bridge C18 column. The mobile phase consisted of a mixture of phosphoric acid 0.05%: acetonitrile (75:25, v/v) with a flow rate of 1 mL/min. The column elute was monitored at 254 nm. The method was linear from 0.2 to 48 mg/L (mean r = 0.9996, n = 10). The observed intra-and inter-day assay imprecision ranged from 2.83% to 8.16% (18.80% at the lower limit of quantification); inaccuracy varied between-0.33% and 8.18%. Mean drug recovery was 99.8% for linezolid and 90.0% for the internal standard (para-toluic acid). The method was found to be precise and accurate and suitable for therapeutic drug monitoring of linezolid in routine clinical practice.
Determination of linezolid in human plasma by high-performance liquid chromatography with ultraviolet detection / D. Cattaneo, S. Baldelli, F. Conti, V. Cozzi, E.G.I. Clementi. - In: THERAPEUTIC DRUG MONITORING. - ISSN 0163-4356. - 32:4(2010), pp. 520-524.
|Titolo:||Determination of linezolid in human plasma by high-performance liquid chromatography with ultraviolet detection|
COZZI, VALERIA (Penultimo)
CLEMENTI, EMILIO GIUSEPPE IGNAZIO (Ultimo)
|Parole Chiave:||HPLC; human plasma; linezolid; pharmacokinetics; therapeutic drug monitoring|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Data di pubblicazione:||2010|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1097/FTD.0b013e3181d5eeee|
|Appare nelle tipologie:||01 - Articolo su periodico|