Previous experiments established that when the unicellular green alga Chlorella NC64A is inoculated with two viruses, usually only one virus replicates in a single cell. That is, the viruses mutually exclude one another. In the current study, we explore the possibility that virus-induced host membrane depolarization, at least partially caused by a virus-encoded K+ channel (Kcv), is involved in this mutual exclusion. Two chlorella viruses, PBCV-1 and NY-2A, were chosen for the study because (i) they can be distinguished by real-time PCR and (ii) they exhibit differential sensitivity to Cs+, a well-known K+ channel blocker. PBCV-1-induced host membrane depolarization, Kcv channel activity and plaque formation are only slightly affected by Cs+, whereas all three NY-2A-induced events are strongly inhibited by Cs+. The addition of one virus 5-15 min before the other results primarily in replication of the first virus. However, if virus NY-2A-induced membrane depolarization of the host is blocked by Cs+, PBCV-1 is not excluded. We conclude that virus-induced membrane depolarization is at least partially responsible for the exclusion phenomenon.
Chlorella viruses prevent multiple infections by depolarizing the host membrane / T. Greiner, F. Frohns, M. Kang, J. Van Etten, A. Käsmann, A. Moroni, B. Hertel, G. Thiel. - In: JOURNAL OF GENERAL VIROLOGY. - ISSN 0022-1317. - 90:6(2009 Aug), pp. 2033-2039.
|Titolo:||Chlorella viruses prevent multiple infections by depolarizing the host membrane|
|Parole Chiave:||green-alga ; restriction endonuclease ; channel protein ; PBCV-1 ; exclusion ; superinfection ; diversity ; cells ; NYS-1 ; KCV|
|Settore Scientifico Disciplinare:||Settore BIO/04 - Fisiologia Vegetale|
|Data di pubblicazione:||ago-2009|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1099/vir.0.010629-0|
|Appare nelle tipologie:||01 - Articolo su periodico|