To assess the possible role of the " brain isoenzyme" of creatine kinase (CK-BB) as an early diagnostic tools for cerebral coenurosis we examined sera from 68 sheep (10-13 months old) from a flock on which cerebral coenurosis was endemical. After sampling, sheep were slaughtered, and neurological lesions were classified as purulent-necrotic migratory lesions or cystic lesions. The discriminating power of CK-BB to diagnose cerebral coenurosis was assessed using a receiver operating characteristic (ROC) curve.The prevalence of cerebral coenurosis was 39.7% (27/68). Migratory lesions were found in 23 sheep (85.1%), cystic lesions in 3 sheep (11.2%) and both lesions were found in 1 sheep (3.7%). The most abundant isoenzymes detected both in controls and in sheep with cerebral coenurosis were CK-MM, Macro-CK2 and CK-BB. No significant differences regarding absolute values of CK-BB were found suggesting that CK-BB is not released in serum during cerebral coenurosis. Conversely, CK-MM was significantly lower (P<0.05) in sheep with cerebral coenurosis compared with controls, inducing a relative increase of the percentage of CK-BB (P<0.05). This latter increase had a good discriminating power (area under the ROC curve: 0.71; P<0.000). Although at its best diagnostic performance (CK-BB higher than 24,4%) sensitivity and specificity were moderate (66.7% and 68.3%, respectively), the likelihood ratio of increased CK-BB was 2.10. The increased percentage of CK-BB can thus be useful in the diagnostic approach to suspected cases of cerebral coenurosis, when the pre-test probability of disease is high.

Brain creatine kinase isoenzyme (CK-BB) as a possible biomarker for the diagnosis in vivo of ovine coenurosis in a naturally infected flock / S. Paltrinieri, A. Varcasia, S. Cazzaniga, A. Giordano, A.P. Pipia, R. Marrosu, A. Scala. - In: SMALL RUMINANT RESEARCH. - ISSN 0921-4488. - 94:1-3(2010 Nov), pp. 180-184. [10.1016/j.smallrumres.2010.07.010]

Brain creatine kinase isoenzyme (CK-BB) as a possible biomarker for the diagnosis in vivo of ovine coenurosis in a naturally infected flock

S. Paltrinieri
Primo
;
A. Giordano;
2010

Abstract

To assess the possible role of the " brain isoenzyme" of creatine kinase (CK-BB) as an early diagnostic tools for cerebral coenurosis we examined sera from 68 sheep (10-13 months old) from a flock on which cerebral coenurosis was endemical. After sampling, sheep were slaughtered, and neurological lesions were classified as purulent-necrotic migratory lesions or cystic lesions. The discriminating power of CK-BB to diagnose cerebral coenurosis was assessed using a receiver operating characteristic (ROC) curve.The prevalence of cerebral coenurosis was 39.7% (27/68). Migratory lesions were found in 23 sheep (85.1%), cystic lesions in 3 sheep (11.2%) and both lesions were found in 1 sheep (3.7%). The most abundant isoenzymes detected both in controls and in sheep with cerebral coenurosis were CK-MM, Macro-CK2 and CK-BB. No significant differences regarding absolute values of CK-BB were found suggesting that CK-BB is not released in serum during cerebral coenurosis. Conversely, CK-MM was significantly lower (P<0.05) in sheep with cerebral coenurosis compared with controls, inducing a relative increase of the percentage of CK-BB (P<0.05). This latter increase had a good discriminating power (area under the ROC curve: 0.71; P<0.000). Although at its best diagnostic performance (CK-BB higher than 24,4%) sensitivity and specificity were moderate (66.7% and 68.3%, respectively), the likelihood ratio of increased CK-BB was 2.10. The increased percentage of CK-BB can thus be useful in the diagnostic approach to suspected cases of cerebral coenurosis, when the pre-test probability of disease is high.
Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria
Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali
nov-2010
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/149086
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 13
  • ???jsp.display-item.citation.isi??? 11
social impact