Toxocara canis (T. canis) is originally a parasite of canine bitches and their pups. The pathogenicity of T. canis infection is enhanced during pregnancy and puppyhood. The aim of this study was to investigate if modification of IFNγ and IL-10 secretion occurs during infection in pregnant dogs and puppies. Analysis of cytokines secreted could let us hypothesize a role for IL-10 and/or IFNγ in T. canis infection. We tested T. canis-specific production of IFNγ and IL-10 by lymphocytes of pregnant dogs and their puppies after in vitro re-exposure to purified excretory/secretory antigen (ESAg) from T. canis. Blood mononuclear cells (BMC) isolated from pregnant dogs and their puppies were cultured in the presence of ESAg. Cultures' supernatants were tested for cytokine levels by ELISA. Results obtained showed that IL-10 concentrations increased during pregnancy in infected animals and in the meantime IFNγ production decreased. In puppyhood, we observed that, IL-10 concentration decreased with the age of puppies mainly in infected animals while IFNγ increased. In conclusion, our data suggests that BMC of infected dogs have a particular modification of IL-10 and IFNγ synthesis. These data could be the basis to design immunotherapeutic approaches.
|Titolo:||Toxocara canis infection induces antigen-specific IL-10 and IFNgamma production in pregnant dogs and their puppies|
|Settore Scientifico Disciplinare:||Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali|
|Data di pubblicazione:||2005|
|Digital Object Identifier (DOI):||10.1016/j.vetimm.2005.08.006|
|Appare nelle tipologie:||01 - Articolo su periodico|