Objective: To investigate if prone position delays the progression of experimental ventilator-induced lung injury, possibly due to a more homogeneous distribution of strain within lung parenchyma. Design: Prospective, randomized, controlled trial. Setting: Animal laboratory of a university hospital. Subjects: Thirty-five Sprague Dawley male rats (weight 257 +/- 45 g). Interventions: Mechanical ventilation in either supine or prone position and computed tomography scan analysis. Measurements: Animals were ventilated in supine (n = 15) or prone (n = 15) position until a similar ventilator-induced lung injury was reached. To do so, experiments were interrupted when respiratory system elastance was 150% of baseline. Ventilator-induced lung injury was assessed as lung wet-to-dry ratio and histology. Time to reach lung injury was considered as a main outcome measure. In five additional animals, computed tomography scans (GE Light Speed QX/I, thickness 1.25 mm, interval 0.6 mm, 100 MA, 100 Kv) were randomly taken at end-expiration and end-inspiration in both positions, and quantitative analysis was performed. Data are shown as mean +/- SD. Measurements and Main Results: Similar ventilator-induced lung injury was reached (respiratory system elastance, wet-to-dry ratio, and histology). The time taken to achieve the target ventilator-induced lung injury was longer with prone position (73 +/- 37 mins vs. 112 +/- 42, supine vs. prone, p = .011). Computed tomography scan analysis performed before lung injury revealed that at end-expiration, the lung was wider in prone position (p = .004) and somewhat shorter (p = .09), despite similar lung volumes (p = .455). Lung density along the vertical axis increased significantly only in supine position (p = .002). Lung strain was greater in supine as opposed to prone position (width strain, 7.8 +/- 1.8% vs. 5.6 +/- 0.9, supine vs. prone, p = .029). Conclusions: Prone position delays the progression of ventilator-induced lung injury. Computed tomography scan analysis suggests that a more homogeneous distribution of strain may be implicated in the protective role of prone position against ventilator-induced lung injury.
|Titolo:||Prone position delays the progression of ventilator-induced lung injury in rats : Does lung strain distribution play a role?|
|Autori interni:||GATTINONI, LUCIANO|
|Parole Chiave:||Acute; Animal experimentation; Lung injury; Prone position; Tomography, spiral computed; Ventilation|
|Settore Scientifico Disciplinare:||Settore MED/41 - Anestesiologia|
|Data di pubblicazione:||2005|
|Digital Object Identifier (DOI):||10.1097/01.CCM.0000150660.45376.7C|
|Appare nelle tipologie:||01 - Articolo su periodico|